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Insecticidal and repellent activity of Hiptage benghalensis L. Kruz (Malpighiaceae) against mosquito vectors

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Abstract

Plant-based insecticides for vector control are urgently needed for Anopheles barbirostris, Culex quinquefasciatus, and Aedes albopictus which are the primary vectors of malaria, lymphatic filariasis, and dengue, respectively, in India and other South East Asian countries. In the present study, larvicidal, adulticidal, and repellent activities of acetone root bark extract of Hiptage benghalensis were tested against the larvae and adults of the three mosquito vectors. The acetone root bark extracts of H. benghalensis was more effective as larvicides with low LC50 (11.15–16.78 ppm) and LT50 (1.25–4.84 h at 200 and 400 ppm) values. Results of log probit analysis (at 95 % confidence level) and regression analysis of crude acetone root bark extract of H. benghalensis revealed that lethal concentration (LC50) values gradually decreased with the exposure periods; lethal time (LT50) decreased with the concentration, and the mortality is positively correlated with the concentration. The order of susceptibility of the three mosquito species was as follows: A. albopictus > A. barbirostris > C. quinquefascitus. Biochemical changes were also evidenced in third instar larvae of three mosquito species following a sublethal exposure for 24 h. The level of sugar, glycogen, lipids, and proteins was significantly (P < 0.05) reduced in larvae treated with H. benghalensis. The acetone root bark extracts of H. benghalensis is less toxic to adults and repelled laboratory-reared female A. barbirostris, A. albopictus, and C. quinquefascitus with the short median protection times of 57.66–135, 72.41–134.16, and 47.66–93 min, respectively. The present investigation proves it as a potent larvicide against A. albopictus, A. barbirostris, and C. quinquefascitus, which can be recommended to control these mosquito species on its breeding site. However, further investigations are needed to confirm the lethal effects of H. benghalensis in field conditions and its impact on the nontarget organisms.

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Acknowledgments

We are thankful to the University Grants Commission, New Delhi, India for the financial assistance to Lalrotluanga (UGC meritorious fellowship) and gratefully acknowledge Mizoram University, Aizawl, Mizoram for providing the necessary facilities. The authors thank CSIR, New Delhi (no. 37(1362)/09/EMR-II dt 3 March 2009) and Directorate of Health Services, Government of Mizoram, Mizoram (D 12030/1/2003–DHS (M)/21 dt. 06 March 2009) for supporting research projects related to mosquito.

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Authors and Affiliations

  1. Department of Zoology, Mizoram University, Aizawl, 796 004, Mizoram, India

    Lalrotluanga & Gurusubramanian Guruswami

  2. Department of Forestry, Mizoram University, Aizawl, 796 004, Mizoram, India

    Lalchawimawii Ngente

  3. Department of Biotechnology, Mizoram University, Aizawl, 796 004, Mizoram, India

    Senthil Kumar Nachimuthu

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  1. Lalrotluanga
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  2. Lalchawimawii Ngente
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  3. Senthil Kumar Nachimuthu
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  4. Gurusubramanian Guruswami
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Correspondence to Gurusubramanian Guruswami.

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Lalrotluanga, Ngente, L., Nachimuthu, S.K. et al. Insecticidal and repellent activity of Hiptage benghalensis L. Kruz (Malpighiaceae) against mosquito vectors. Parasitol Res 111, 1007–1017 (2012). https://doi.org/10.1007/s00436-012-2925-7

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