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The roles of extracellular polymeric substances of Pandoraea sp. XY-2 in the removal of tetracycline

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Abstract

In this study, the roles of extracellular polymeric substances (EPSs) excreted by Pandoraea sp. XY-2 in the removal of tetracycline (TC) were investigated. In the early stage, TC in the solution was mainly removed by the adsorption of EPSs, which accounted for 20% of TC. Thereafter, large amount of TC was transported into the intracellular and biodegraded. EPSs was extracted and the contents of polyprotein and polysaccharides reached their maximum values (30.84 mg/g and 11.15 mg/g) in the first four days. Fourier transform infrared spectroscopy analysis revealed that hydroxyl, methylidyne, methylene and amide I groups in EPSs participated in the adsorption of TC. Furthermore, three-dimensional excitation-emission matrix fluorescence spectroscopy analysis revealed that TC caused the quenching of EPSs fluorescent groups. The quenching mechanism was attributed to static quenching and protein-like substances in EPSs from Pandoraea sp. XY-2 dominated the TC adsorption process. Bioinformatic analysis of Pandoraea sp. XY-2 genome identified multiple genes involved in exopolysaccharide synthesis and EPSs formation. The insights gained in this study might provide a better understanding about the adsorption process of EPSs in tetracycline-contaminated environment.

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Acknowledgements

X.W. (Xiaoyan Wu) conducted the experiment, analyzed data and drafted the manuscript; X.Z., Y.G., and H.Z. were involved in the experiment; X.W. (Xueling Wu), L.S., and W.Z. contributed to the conceptualization and project administration, manuscript reviewing and editing. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the Fundamental Research Funds for the Central Universities of Central South University (no. 2019zzts175), the National Natural Science Foundation of China (nos. 31470230, 51320105006, 51604308), the Youth Talent Foundation of Hunan province of China (no. 2017RS3003), Natural Science Foundation of Hunan Province of China (no. 2018JJ2486), Fundamental Research Funds for the Central Universities of Central South University (no. 2018zzts767).

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Authors and Affiliations

  1. School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China

    Xueling Wu, Xiaoyan Wu, Xiangyu Zhou, Yichao Gu, Han Zhou, Li Shen & Weimin Zeng

  2. Key Laboratory of Biometallurgy, Ministry of Education, Changsha, 410083, China

    Xueling Wu, Li Shen & Weimin Zeng

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  1. Xueling Wu
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Correspondence to Weimin Zeng.

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Wu, X., Wu, X., Zhou, X. et al. The roles of extracellular polymeric substances of Pandoraea sp. XY-2 in the removal of tetracycline. Bioprocess Biosyst Eng 43, 1951–1960 (2020). https://doi.org/10.1007/s00449-020-02384-8

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