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Oxidant/antioxidant status and hyperfiltration in young patients with type 1 diabetes mellitus

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Abstract

Diabetic nephropathy (DN), a major cause of morbidity and mortality in diabetes, will develop within a subset of type 1 diabetes mellitus (T1DM) patients, and oxidative stress has been implicated in its pathogenesis. To investigate the relationship between indicators of early DN stages (hyperfiltration estimated by creatinine clearance ≥150 ml/min per 1.73 m2, microalbuminuria) and oxidative stress, a prospective study was conducted in 29 T1DM patients (age 13.89 ± 4.61 years) and 18 control subjects (age 13.23 ± 3.99 years). Blood samples were collected to assay for biomarkers of oxidative stress (malondialdehyde and carbonyl groups) and antioxidants (glutathione peroxidase, reduced glutathione, α-tocopherol, and β-carotene). With respect to control subjects, in T1DM patients, an increase was found in biomarkers of oxidative stress (p < 0.05), mainly due to the group of subjects with hyperfiltration, and a decrease in the ratio α-tocopherol/lipids (p < 0.05). In multiple regression analyses, age at disease onset, glycated hemoglobin, microalbuminuria, and oxidative stress biomarkers remained as explicative variables of hyperfiltration (R 2 adjusted = 0.731, p = 0.000). These findings support the importance of the oxidative damage to lipids and proteins, which is linked to hyperfiltration and which could contribute to the development of DN in patients with T1DM.

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Acknowledgements

We appreciate the collaboration of the parents whose children participated in this study.

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Authors and Affiliations

  1. Department of Pediatrics, Dr. Peset University Hospital, Valencia, Spain

    Roberto Hernández-Marco, Pilar Codoñer-Franch, Sara Pons Morales & Cristina del Castillo Villaescusa

  2. Department of Pediatrics, Obstetrics and Gynecology, Faculty of Medicine and Odontology, University of Valencia, Valencia, Spain

    Roberto Hernández-Marco, Pilar Codoñer-Franch, Laura Boix García & Victoria Valls Bellés

  3. Facultad de Medicina y Odontología, Departamento POG, Avenida Blasco Ibáñez, 15, 46010, Valencia, Spain

    Pilar Codoñer-Franch

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  1. Roberto Hernández-Marco
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  2. Pilar Codoñer-Franch
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  3. Sara Pons Morales
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  4. Cristina del Castillo Villaescusa
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  5. Laura Boix García
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Correspondence to Pilar Codoñer-Franch.

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Hernández-Marco, R., Codoñer-Franch, P., Pons Morales, S. et al. Oxidant/antioxidant status and hyperfiltration in young patients with type 1 diabetes mellitus. Pediatr Nephrol 24, 121–127 (2009). https://doi.org/10.1007/s00467-008-0961-4

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  • DOI: https://doi.org/10.1007/s00467-008-0961-4

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