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Effect of auxins on adventitious rooting from stem cuttings of candidate plus tree Pongamia pinnata (L.), a potential biodiesel plant

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Abstract

Pongamia pinnata, commercially important tree species used to produce biofuels, is known for its multipurpose benefits and its role in agro-forestry. Present study examines the amenability of vegetative propagation and effect of maturation in candidate plus tree P. pinnata through rooting of stem cuttings treated with varying concentrations and combinations of auxins. The performance of the cuttings was evaluated using SAS GLM software and the data were analyzed as a one-way classified data with and without sub sampling for inferring auxin concentration that can be included in programmes aimed at genetic improvement of the tree species. All auxin treatments promoted sprouting and at lower concentrations triggered/enhanced rooting of cuttings. The effectiveness was in the order of IBA > NAA > IAA when applied singly. IBA at 4.92 mM was found to be most effective where rooting percentage and number of roots were significantly higher (P < 0.01) than in control. However higher concentrations of auxins above 7 mM in general inhibited the rooting of cuttings. The interaction among auxins was found to be effective in root induction and differentiation and the most stimulating effects were observed in three-component mixture. The effect of other cutting characteristics such as juvenility and cutting position on rooting is also discussed.

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Abbreviations

CPT:

Candidate plus tree

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

NAA:

Naphthalene acetic acid

NG:

North Guwahati

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Acknowledgments

VK thanks Ministry of Human Resources Development (MHRD), Government of India for fellowship. Thanks to the Forest Department officials of Sila Forest, North Guwahati for kind supply of study material and for provision of technical staff. LR acknowledges funding by the Department of Science and Technology, Government of India SERC Fast Track Young Scientist Scheme.

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Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, 781 039, India

    Vigya Kesari & Latha Rangan

  2. Crop Research Informatics Laboratory, International Rice Research Institute, DAPO Box 7777, Metro Manila, The Philippines

    Anitha Krishnamachari

Authors
  1. Vigya Kesari
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  2. Anitha Krishnamachari
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  3. Latha Rangan
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Correspondence to Latha Rangan.

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Communicated by F. Canovas.

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Kesari, V., Krishnamachari, A. & Rangan, L. Effect of auxins on adventitious rooting from stem cuttings of candidate plus tree Pongamia pinnata (L.), a potential biodiesel plant. Trees 23, 597–604 (2009). https://doi.org/10.1007/s00468-008-0304-x

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  • DOI: https://doi.org/10.1007/s00468-008-0304-x

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