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Impact of the laurina mutation in Coffea arabica L. on semi-dwarfism, cell number and hormonal profiles in hypocotyls of seedlings growing under daylight

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Abstract

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Hypocotyl semi-dwarfism in BP was only due to less cell number. Daylight was required to inhibit cell division in the mutant. ABA, IAA and cytokinins would be involved.

Abstract

Coffea arabica ‘Laurina’ is a natural mutant of Coffea arabica ‘Bourbon’ (B) and is known under the trade name ‘Bourbon Pointu’ (BP). Under daylight, the laurina mutation leads to pleiotropic effects, including semi-dwarf hypocotyls. At the opposite, semi-dwarfism of BP seedlings disappeared under darkness conditions. The first step was to describe the morphological impact of the mutation in seedlings growing under daylight by comparison with seedlings growing under darkness. As the hypocotyl length was mainly affected, the second step was to investigate histological modifications in the organ comparing B and BP seedlings growing under daylight. Result of this investigation indicated that the mutation does not impact on cell length. Moreover, cytometry analyses showed absence of endoreduplication. Actually, the mutation influenced the cell number and this effect appeared before the 40th day after sowing. The length difference of hypocotyls between B and BP was due to lower cell number in BP, indicating possible involvement of phytohormones. Investigations showed the decrease of cytokinin and auxin levels in BP compared to B, while the cytokinin/auxin ratio remained constant in both varieties. By contrast, abscisic acid content increased in BP. Concurrently these results indicate the lowering of cell division, due to the mutation.

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Author contribution statement

Sophie Adler: she carried out experiments leading to histological and hormonal approaches. She did cytometry experiments, histological measures, statistical analyses, and paper writing. Jean-Luc Verdeil: he coached histological approaches and participated in paper correction. Geneviève Conéjéro: she coached microscope use, and participated in paper correction. Irina L. Zaharia: she coached hormonal dosages in the laboratory and participated in paper correction and English improvement. Amélie Sarrazin: she coached cytometry experiment. Julien Hoareau: he was at the origin of the paper, emphasizing the role of daylight on seedling morphology in the mutant. Isabelle Fock-Bastide: she participated in the paper writing. Michel Noirot: he participated in statistical analyses and paper writing.

Acknowledgments

This study was financially supported by the European Union, the Conseil Régional of Réunion and the French Institut pour la Recherche et le Développement (IRD). The authors thank Dr. Xiumei Han, Vera Cekic and Dang Van for excellent technical assistance. Authors also thank Pr. Charrier for its helpful comments.

Conflict of interest

The authors declare that they have no conflict of interest

Author information

Authors and Affiliations

  1. Pôle de Protection des Plantes, Université de la Réunion, UMR Cirad-Université PVBMT, 97410, St Pierre, Réunion, France

    Sophie Adler, Julien Hoareau & Isabelle Fock-Bastide

  2. Laboratoire PHIV,UMR AGAP INRA/CIRAD/SupAgro, 34000, Montpellier, France

    Jean-Luc Verdeil

  3. Laboratoire PHIV, UMR BPMP INRA/CNRS/SupAgro/UM II, 34000, Montpellier, France

    Geneviève Conejero

  4. Aquatic and Crop Resource Development, National Research Council of Canada, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada

    Irina L. Zaharia

  5. RIO Imaging platform, 34000, Montpellier, France

    Amélie Sarrazin

  6. Pôle de Protection des Plantes, IRD, UMR Cirad-Université PVBMT, 97410, St Pierre, Réunion, France

    Michel Noirot

Authors
  1. Sophie Adler
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  2. Jean-Luc Verdeil
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  4. Irina L. Zaharia
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  6. Julien Hoareau
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  7. Isabelle Fock-Bastide
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  8. Michel Noirot
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Corresponding author

Correspondence to Michel Noirot.

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Communicated by F. Canovas.

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Adler, S., Verdeil, JL., Conejero, G. et al. Impact of the laurina mutation in Coffea arabica L. on semi-dwarfism, cell number and hormonal profiles in hypocotyls of seedlings growing under daylight. Trees 29, 1197–1205 (2015). https://doi.org/10.1007/s00468-015-1200-9

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  • DOI: https://doi.org/10.1007/s00468-015-1200-9

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