Abstract
Although the seed remains small in size during the initial stage of seed development (the lag phase), several studies indicate that environment and assimilate supply level manipulations during the lag phase affect the final seed size. However, the manipulations were not only at the lag phase, making it difficult to understand the specific role of the lag phase in final seed size determination. It also remained unclear whether environmental cues are sensed by plants and regulate seed development or if it is simply the assimilate supply level, changed by the environment, that affects the subsequent seed development. We investigated soybean (Glycine max L. Merr.) seed phenotypes grown in a greenhouse using different source-sink manipulations (shading and removal of flowers and pods) during the lag phase. We show that assimilate supply is the key factor controlling flower and pod abortion and that the assimilate supply during the lag phase affects the subsequent potential seed growth rate during the seed filling phase. In response to low assimilate supply, plants adjust flower/pod abortion and lag phase duration to supply the minimum assimilate per pod/seed. Our results provide insight into the mechanisms whereby the lag phase is crucial for seed development and final seed size potential, essential parameters that determine yield.
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References
Berger F, Grini PE, Schnittger A (2006) Endosperm: an integrator of seed growth and development. Curr Opin Plant Biol 9:664–670. https://doi.org/10.1016/j.pbi.2006.09.015
Borrás L, Slafer GA, Otegui ME (2004) Seed dry weight response to source–sink manipulations in wheat, maize and soybean: a quantitative reappraisal. Field Crops Res 86:131–146. https://doi.org/10.1016/j.fcr.2003.08.002
Braselton JP, Wilkinson MJ, Clulow SA (1996) Feulgen staining of intact plant tissues for confocal microscopy. Biotech Histochem 71:84–87. https://doi.org/10.3109/10520299609117139
Brown RC, Lemmon BE, Olsen O-A (1996) Development of the endosperm in rice (Oryza sativa L.): cellularization. J Plant Res 109:301–313. https://doi.org/10.1007/BF02344477
Calderini DF, Abeledo LG, Savin R, Slafer GA (1999) Effect of temperature and carpel size during pre-anthesis on potential grain weight in wheat. J Agric Sci 132:453–459. https://doi.org/10.1017/S0021859699006504
Chamberlin MA, Horner HT, Palmer RG (1994) Early endosperm, embryo, and ovule development in glycine max (L.) Merr. Int J Plant Sci 155:421–436
Chiluwal A, Kawashima T, Salmeron M (2021) Soybean seed weight responds to increases in assimilate supply during late seed-fill phase. J Crop Improv 1–17. https://doi.org/10.1080/15427528.2021.1943732
Chowdhury W (1978) The effect of temperature on kernel development in cereals [wheat, rice, sorghum]. Aust J Agric Res 29:205–223. https://doi.org/10.1071/AR9780205
Dute RR, Peterson CM (1992) Early endosperm development in ovules of soybean, glycine max (L) Merr. (Fabaceae)*. Ann Bot 69:263–271. https://doi.org/10.1093/oxfordjournals.aob.a088339
Egli DB (2004) Seed-fill duration and yield of grain crops. Adv Agron 83:243–279. https://doi.org/10.1016/s0065-2113(04)83005-0
Egli DB (2010) soybean reproductive sink size and short-term reductions in photosynthesis during flowering and pod set. Crop Sci 50:1971–1977. https://doi.org/10.2135/cropsci2009.09.0518
Egli DB, Bruening WP (2001) Source-sink relationships, seed sucrose levels and seed growth rates in soybean. Ann Bot 88:235–242. https://doi.org/10.1006/anbo.2001.1449
Egli DB, Fraser J, Leggett JE, Poneleit CG (1981) Control of seed growth in soya beans [glycine max (L.) Merrill]. Ann Bot 48:171–176. https://doi.org/10.1093/oxfordjournals.aob.a086110
Egli DB, Wardlaw IF (1980) Temperature response of seed growth characteristics of Soybeans1. Agron J 72:560–564. https://doi.org/10.2134/agronj1980.00021962007200030036x
Egli DB, Ramseur EL, Zhen-wen Y, Sullivan CH (1989) Source-sink alterations affect the number of cells in soybean cotyledons. Crop Sci 29:732–735. https://doi.org/10.2135/cropsci1989.0011183X002900030039x
Egli DB, Guffy RD, Meckel LW, Leggett JE (1985) The effect of source-sink alterations on soybean seed growth. Ann Bot UK
Egli D (2017) Seed Biology and Yield of Grain Crops. CAB Int Oxfs. https://doi.org/10.1079/9781780647708.0000
Fehr W, Caviness C (1977) Stages of soybean development. Spec Rep 87
Gambín BL, Borrás L, Otegui ME (2006) Source–sink relations and kernel weight differences in maize temperate hybrids. Field Crops Res 95:316–326. https://doi.org/10.1016/j.fcr.2005.04.002
Gao X, Francis D, Ormrod JC, Bennett MD (1992) Changes in cell number and cell division activity during endosperm development in allohexaploid wheat, Triticum aestivum L. J Exp Bot 43:1603–1609. https://doi.org/10.1093/jxb/43.12.1603
Guldan SJ, Brun WA (1985) Relationship of cotyledon cell number and seed respiration to soybean seed growth1. Crop Sci 25:815–819. https://doi.org/10.2135/cropsci1985.0011183X002500050021x
Heitholt JJ, Egli DB, Leggett JE (1986) Characteristics of reproductive abortion in soybean1. Crop Sci 26:589–595. https://doi.org/10.2135/cropsci1986.0011183X002600030034x
Jiang H, Egli DB (1995) Soybean seed number and crop growth rate during flowering. Agron J 87:264–267. https://doi.org/10.2134/agronj1995.00021962008700020020x
Jones RJ, Roessler J, Ouattar S (1985) Thermal environment during endosperm cell division in maize: effects on number of endosperm cells and starch granules1. Crop Sci 25:830–834. https://doi.org/10.2135/cropsci1985.0011183X002500050025x
Kantolic AG, Slafer GA (2005) Reproductive development and yield components in indeterminate soybean as affected by post-flowering photoperiod. Field Crops Res 93:212–222. https://doi.org/10.1016/j.fcr.2004.10.001
Kantolic AG, Peralta GE, Slafer GA (2013) Seed number responses to extended photoperiod and shading during reproductive stages in indeterminate soybean. Eur J Agron 51:91–100. https://doi.org/10.1016/j.eja.2013.07.006
Lafon-Placette C, Johannessen IM, Hornslien KS et al (2017) Endosperm-based hybridization barriers explain the pattern of gene flow between Arabidopsis lyrata and Arabidopsis arenosa in Central Europe. Proc Natl Acad Sci 114:E1027–E1035. https://doi.org/10.1073/pnas.1615123114
Mason MG, Ross JJ, Babst BA et al (2014) Sugar demand, not auxin, is the initial regulator of apical dominance. Proc Natl Acad Sci 111:6092–6097. https://doi.org/10.1073/pnas.1322045111
Nico M, Miralles DJ, Kantolic AG (2015) Post-flowering photoperiod and radiation interaction in soybean yield determination: Direct and indirect photoperiodic effects. Field Crops Res 176:45–55. https://doi.org/10.1016/j.fcr.2015.02.018
Nico M, Mantese AI, Miralles DJ, Kantolic AG (2016) Soybean fruit development and set at the node level under combined photoperiod and radiation conditions. J Exp Bot 67:365–377. https://doi.org/10.1093/jxb/erv475
Olsen O-A, Linnestad C, Nichols SE (1999) Developmental biology of the cereal endosperm. Trends Plant Sci 4:253–257. https://doi.org/10.1016/S1360-1385(99)01431-4
Orozco-Arroyo G, Paolo D, Ezquer I, Colombo L (2015) Networks controlling seed size in Arabidopsis. Plant Reprod 28:17–32. https://doi.org/10.1007/s00497-015-0255-5
Sabelli PA, Larkins BA (2009) The development of endosperm in grasses. Plant Physiol 149:14–26. https://doi.org/10.1104/pp.108.129437
Scott RJ, Spielman M, Bailey J, Dickinson HG (1998) Parent-of-origin effects on seed development in Arabidopsis thaliana. Development 125:3329–3341. https://doi.org/10.1242/dev.125.17.3329
Tacarindua CRP, Shiraiwa T, Homma K et al (2013) The effects of increased temperature on crop growth and yield of soybean grown in a temperature gradient chamber. Field Crops Res 154:74–81. https://doi.org/10.1016/j.fcr.2013.07.021
Acknowledgements
We thank Drs. Dennis B. Egli and Anthony J. Clark, and Ms. Ashwini Shivakumar for their critical comments on this manuscript.
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PB, JT, MS, and TK were supported by USDA-NIFA ELI-REEU 2017-06637. TK was supported by USDA-NIFA Hatch Program 1014280.
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Ali, M.F., Brown, P., Thomas, J. et al. Effect of assimilate competition during early seed development on the pod and seed growth traits in soybean. Plant Reprod 35, 179–188 (2022). https://doi.org/10.1007/s00497-022-00439-2
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DOI: https://doi.org/10.1007/s00497-022-00439-2