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Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations

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Abstract

The authors report on a fluorometric method for the rapid detection of BRCA1, CFRT and MRP3 gene mutations. These are associated with breast cancer, cystic fibrosis and autoimmune hepatitis diseases, respectively. Carbon nanodots with blue fluorescence (with excitation/emission maxima at 340/440 nm) were synthesized and characterized, and their interactions with DNA were investigated. Changes in the fluorescence intensity following interaction with ssDNA and dsDNA were used for specific DNA sequence of BRCA1, CFRT and MRP3 genes detection. The response to DNAs is linear up to 200 nM and the detection limit is 270 pM. The assay selectivity allows the detection of single gene mutations. Under optimum conditions, the assay can rapidly discriminate between wild type and mutated samples.

Schematic representation of fluorescence assay for rapid detection of gene mutation based on fluorescent carbon nanodots.

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Acknowledgements

CAM projects: TRANSNANOAVANSENS-CM (S2018/NMT-4349) and MAD2D-CM Program. MEIC projects: CTQ2017-84309-C2-1-R and MAT2015-71879-P. We thank the Confocal Microscopy and Flow Cytometry Services of CBMSO.

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Authors and Affiliations

  1. Analytical Chemistry Department, Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049, Madrid, Spain

    Tania García-Mendiola, Cristina Garcia Elosegui, Iria Bravo, Félix Pariente & Encarnación Lorenzo

  2. Instituto Madrileño de Estudios Avanzados (IMDEA) Nanociencia, Faraday, 9, 28049, Madrid, Spain

    Tania García-Mendiola, Iria Bravo, Félix Pariente, Alejandra Jacobo-Martin, Cristina Navio, Isabel Rodriguez, Reinhold Wannemacher & Encarnación Lorenzo

Authors
  1. Tania García-Mendiola
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  2. Cristina Garcia Elosegui
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  3. Iria Bravo
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  4. Félix Pariente
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  5. Alejandra Jacobo-Martin
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  6. Cristina Navio
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  7. Isabel Rodriguez
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  8. Reinhold Wannemacher
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  9. Encarnación Lorenzo
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Correspondence to Encarnación Lorenzo.

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García-Mendiola, T., Elosegui, C.G., Bravo, I. et al. Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations. Microchim Acta 186, 293 (2019). https://doi.org/10.1007/s00604-019-3386-9

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  • DOI: https://doi.org/10.1007/s00604-019-3386-9

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