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Rapid and specific detection nanoplatform of serum exosomes for prostate cancer diagnosis

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Abstract

Tumor exosomes that inherit specific molecules from their parent cells are emerging as ideal biomarkers in cancer diagnostics. Most currently available exosome isolation and detection methods are time-consuming and non-specific; thus, rapid and specific exosome detection methods are needed both clinically and in research. Here, a dual-functional platform is reported composed of reversible conjunction and “off-on” signal responses. Fe3O4@SiO2@TiO2 particles with high affinity were applied to capture exosomes, and model exosomes could be isolated from solution within 20 min with a capture efficiency of 91.5%. An “on-off” fluorescence response PSMA aptasensor was constructed with improved selectivity to detect tumor exosomes by recording the fluorescence intensity with λex/em = 557/580 nm. The standard curve for detecting tumor exosomes with the aptasensor was calculated as y = 371.7x + 66.17, ranging from 0.05 to 1 × 104 particles/μL, with R2 = 0.9737, and a detection limit of 5 × 102 particles/μL in solution. This method was successfully applied to clinical samples, and the results showed better performance in distinguishing prostate cancer patients and healthy samples than the traditional nanoparticle-tracking analysis (NTA) method. This rapid and accurate detection method for prostate cancer may aid in rapid clinical diagnosis.

Graphical abstract

Integrating quickly TiO2-based isolation with sensitive and specific “on-off” detection of PCa exosomes.

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Funding

This work was supported by the National Key Research and Development Plan of China (2018YFF0212501) and National Natural Science Foundation of China (31470786).

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Authors and Affiliations

  1. Department of Chemistry, Fudan University, Shanghai, 200438, China

    Qiaoyu Li, Yanlin Wang, Liang Qiao & Hui Chen

  2. Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, Zhejiang, China

    Ling Ling, Chuanfan Ding & Shaoning Yu

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  1. Qiaoyu Li
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  2. Yanlin Wang
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  3. Ling Ling
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  4. Liang Qiao
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  6. Chuanfan Ding
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  7. Shaoning Yu
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The manuscript was written through contributions of all authors.

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Correspondence to Chuanfan Ding or Shaoning Yu.

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Li, Q., Wang, Y., Ling, L. et al. Rapid and specific detection nanoplatform of serum exosomes for prostate cancer diagnosis. Microchim Acta 188, 283 (2021). https://doi.org/10.1007/s00604-021-04934-7

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  • DOI: https://doi.org/10.1007/s00604-021-04934-7

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