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Identification of a disease complex involving a novel monopartite begomovirus with beta- and alphasatellites associated with okra leaf curl disease in Oman

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Abstract

Okra leaf curl disease (OLCD) is an important viral disease of okra in tropical and subtropical areas. The disease is caused by begomovirus-satellite complexes. A begomovirus and associated betasatellite and alphasatellite were identified in symptomatic okra plants from Barka, in the Al-Batinah region of Oman. Analysis of the begomovirus sequences showed them to represent a new begomovirus most closely related to cotton leaf curl Gezira virus (CLCuGeV), a begomovirus of African origin. The sequences showed less than 85 % nucleotide sequence identity to CLCuGeV isolates. The name okra leaf curl Oman virus (OLCOMV) is proposed for the new virus. Further analysis revealed that the OLCOMV is a recombinant begomovirus that evolved by the recombination of CLCuGeV isolates with tomato yellow leaf curl virus-Oman (TYLCV-OM). An alpha- and a betasatellite were also identified from the same plant sample, which were also unique when compared to sequences available in the databases. However, although the betasatellite appeared to be of African origin, the alphasatellite was most closely related to alphasatellites originating from South Asia. This is the first report of a begomovirus-satellite complex infecting okra in Oman.

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Acknowledgements

This study was supported by research grant number ORG/EBR/09/03, funded by The Research Council, Oman to AJK. RWB is supported by the Higher Education Commission, Government of Pakistan, under the ‘‘Foreign Faculty Hiring Program.’’

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Authors and Affiliations

  1. Department of Crop Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khod, Muscat, 123, Oman

    Sohail Akhtar & Akhtar J. Khan

  2. School of Life Sciences, Central University of Gujrat, Gandhinagar, India

    Achuit S. Singh

  3. National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan

    Rob W. Briddon

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  1. Sohail Akhtar
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  2. Akhtar J. Khan
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Correspondence to Akhtar J. Khan.

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Akhtar, S., Khan, A.J., Singh, A.S. et al. Identification of a disease complex involving a novel monopartite begomovirus with beta- and alphasatellites associated with okra leaf curl disease in Oman. Arch Virol 159, 1199–1205 (2014). https://doi.org/10.1007/s00705-013-1926-x

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