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Molecular characterization of a novel ssRNA ourmia-like virus from the rice blast fungus Magnaporthe oryzae

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Abstract

In this study we characterize a novel positive and single stranded RNA (ssRNA) mycovirus isolated from the rice field isolate of Magnaporthe oryzae Guy11. The ssRNA contains a single open reading frame (ORF) of 2,373 nucleotides in length and encodes an RNA-dependent RNA polymerase (RdRp) closely related to ourmiaviruses (plant viruses) and ourmia-like mycoviruses. Accordingly, we name this virus Magnaporthe oryzae ourmia-like virus 1 (MOLV1). Although phylogenetic analysis suggests that MOLV1 is closely related to ourmia and ourmia-like viruses, it has some features never reported before within the Ourmiavirus genus. 3’ RLM-RACE (RNA ligase-mediated rapid amplification of cDNA ends) and extension poly(A) tests (ePAT) suggest that the MOLV1 genome contains a poly(A) tail whereas the three cytosine and the three guanine residues present in 5’ and 3’ untranslated regions (UTRs) of ourmia viruses are not observed in the MOLV1 sequence. The discovery of this novel viral genome supports the hypothesis that plant pathogenic fungi may have acquired this type of viruses from their host plants.

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Acknowledgements

A.S. is funded by the Spanish Research council (grant ref. BIO2014-53211-R), the EC (REA grant agreement n. 304039) and the Community of Madrid (grant ref. S2013/ABI-2734).

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Authors and Affiliations

  1. Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM), Pozuelo de Alarcón, 28223, Madrid, Spain

    Adriana Illana, Marco Marconi, Julio Rodríguez-Romero, Mark D. Wilkinson, Maria Ángeles Ayllón & Ane Sesma

  2. Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, UPM, 28040, Madrid, Spain

    Adriana Illana, Marco Marconi, Julio Rodríguez-Romero, Mark D. Wilkinson, Maria Ángeles Ayllón & Ane Sesma

  3. School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK

    Ping Xu & Tamas Dalmay

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  1. Adriana Illana
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  2. Marco Marconi
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  3. Julio Rodríguez-Romero
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Correspondence to Ane Sesma.

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Illana, A., Marconi, M., Rodríguez-Romero, J. et al. Molecular characterization of a novel ssRNA ourmia-like virus from the rice blast fungus Magnaporthe oryzae . Arch Virol 162, 891–895 (2017). https://doi.org/10.1007/s00705-016-3144-9

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