Abstract
A novel strain of Botryosphaeria dothidea chrysovirus 1 was identified. It encodes a shortened RNA-dependent RNA polymerase and an elongated coat protein, and it might cause hypovirulence of the host fungal strain.
References
Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384
Nuss DL (2005) Hypovirulence: mycoviruses at the fungal-plant interface. Nat Rev Microbiol 3:632–642
Pearson MN, Beever RE, Boine B, Arthur K (2009) Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol Plant Pathol 10:115–128
Aoki N, Moriyama H, Kodama M, Arie T, Teraoka T, Fukuhara T (2009) A novel mycovirus associated with four double-stranded RNAs affects host fungal growth in Alternaria alternata. Virus Res 140:179–187
Cai G, Myers K, Hillman BI, Fry WE (2009) A novel virus of the late blight pathogen, Phytophthora infestans, with two RNA segments and a supergroup 1 RNA-dependent RNA polymerase. Virology 392:52–61
Chiba S, Salaipeth L, Lin YH, Sasaki A, Kanematsu S, Suzuki N (2009) A novel bipartite double-stranded RNA Mycovirus from the white root rot Fungus Rosellinia necatrix: molecular and biological characterization, taxonomic considerations, and potential for biological control. J Virol 83:12801–12812
Liu H, Fu Y, Jiang D, Li G, Xie J, Peng Y, Yi X, Ghabrial SA (2009) A novel mycovirus that is related to the human pathogen hepatitis E virus and rubi-like viruses. J Virol 83:1981–1991
Ghabrial SA, Caston JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368
Newhouse JR, Hoch HC, Macdonald WL (1983) The ultrastructure of Endothia parasitica—Comparison of a virulent with a hypovirulent isolate. Can J Bot 61:389–399
Boland GJ (1992) Hypovirulence and double-stranded-RNA in Sclerotinia sclerotiorum. Can J Plant Pathol 14:10–17
Bottacin AM, Levesque CA, Punja ZK (1994) Characterization of dsRNA in Chalara elegans and effects on growth and virulence. Phytopathology 84:303–312
Anagnostakis SL, Day PR (1979) Hypovirulence conversion in Endothia parasitica. Phytopathology 69:1226–1229
Nuss DL (1992) Biological control of chestnut blight: an example of virus-mediated attenuation of fungal pathogenesis. Microbiol Rev 56:561–576
Guo LY, Li JY, Li BH, Zhang XZ, Zhou ZQ, Li GX, Wang YZ, Li XJ, Huang LL, Sun GY, Wen YD (2009) Investigation on the occurrence and chemical control of Botryosphaeria canker of apple in China. Plant Protect 35:120–123
Weaver DJ (1974) Gummosis disease of peach trees caused by Botryosphaeria dothidea. Phytopathology 64:1429–1432
Michailides TJ, Morgan DP, Felts D (1998) Spread of Botryosphaeria dothidea in central California pistachio orchards. Acta Hortic. 470:582–591
Phillips AJ, Rumbos IC, Alves A, Correia A (2005) Morphology and phylogeny of Botryosphaeria dothidea causing fruit rot of olives. Mycopathologia 159:433–439
Sutton TB (1981) Production and dispersal of ascospores and conidia by Physalospora obtusa and Botryosphaeria dothidea in apple orchards. Phytopathology 71:584–589
Garibaldi A, Bertetti D, Poli A, Gullino ML (2012) First report of fruit rot in pear caused by Botryosphaeria dothidea in Italy. Plant Dis 96:910
Wang L, Jiang J, Wang Y, Hong N, Zhang F, Xu W, Wang G (2014) Hypovirulence of the phytopathogenic fungus Botryosphaeria dothidea: association with a coinfecting chrysovirus and a partitivirus. J Virol 88:7517–7527
Zhai L, Hong N, Zhang M, Wang G (2015) Complete dsRNA sequence of a novel victorivirus isolated from the pear stem wart fungus Botryosphaeria dothidea. Arch Virol 160:613–616
Jiang DH, Ghabrial SA (2004) Molecular characterization of Penicillium chrysogenum virus: reconsideration of the taxonomy of the genus Chrysovirus. J Gen Virol 85:2111–2121
Urayama S, Kato S, Suzuki Y, Aoki N, Le MT, Arie T, Teraoka T, Fukuhara T, Moriyama H (2010) Mycoviruses related to chrysovirus affect vegetative growth in the rice blast fungus Magnaporthe oryzae. J Gen Virol 91:3085–3094
Potgieter AC, Steele AD, van Dijk AA (2002) Cloning of complete genome sets of six dsRNA viruses using an improved cloning method for large dsRNA genes. J Gen Virol 83:2215–2223
Suzuki N, Supyani S, Maruyama K, Hillman BI (2004) Complete genome sequence of Mycoreovirus-1/Cp9B21, a member of a novel genus within the family Reoviridae, isolated from the chestnut blight fungus Cryphonectria parasitica. J Gen Virol 85:3437–3448
Xia ZH, Peng J, Li YQ, Chen L, Li SA, Zhou T, Fan ZF (2014) Characterization of small interfering RNAs derived from Sugarcane Mosaic Virus in infected maize plants by deep sequencing. PloS One 9(5):e97013
Chenna R, Sugawara H, Koike T, Lopez R, Gibson TJ, Higgins DG, Thompson JD (2003) Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res 31:3497–3500
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Yaegashi H, Kanematsu S, Ito T (2012) Molecular characterization of a new hypovirus infecting a phytopathogenic fungus, Valsa ceratosperma. Virus Res 165:143–150
Tang W, Ding Z, Zhou ZQ, Wang YZ, Guo LY (2012) Phylogenetic and pathogenic analyses show that the causal agent of apple ring rot in China is Botryosphaeria dothidea. Plant Dis 96:486–496
Ghabrial SA (2012) Family Chrysoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus Taxonomy: ninth report of the international committee on the taxonomy of viruses. Elsevier Academic Press, San Diego, pp 509–513
Lin YH, Chiba S, Tani A, Kondo H, Sasaki A, Kanematsu S, Suzuki N (2012) A novel quadripartite dsRNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix. Virology 426:42–50
Wu M, Jin F, Zhang J, Yang L, Jiang D, Li G (2012) Characterization of a novel bipartite double-stranded RNA mycovirus conferring hypovirulence in the phytopathogenic fungus Botrytis porri. J Virol 86:6605–6619
Blum C, Gotsch S, Heinze C (2016) Duplications in the 3’ termini of three segments of Fusarium graminearum virus China 9. Arch Virol
Wickner RB (1996) Double-stranded RNA viruses of Saccharomyces cerevisiae. Microbiol Rev 60:250–265
Attoui H, de Micco P, de Lamballerie X (1997) Complete nucleotide sequence of Colorado tick fever virus segments M6, S1 and S2. J Gen Virol 78(11):2895–2899
Wei CZ, Osaki H, Iwanami T, Matsumoto N, Ohtsu Y (2003) Molecular characterization of dsRNA segments 2 and 5 and electron microscopy of a novel reovirus from a hypovirulent isolate, W370, of the plant pathogen Rosellinia necatrix. J Gen Virol 84:2431–2437
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The authors declare that they have no conflict of interest. This study did not include experiments with human participants or animals performed by any of the authors.
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This work was supported by the Earmarked Fund for Modern Agro-Industry Technology Research System (CARS-28).
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705_2017_3320_MOESM1_ESM.pdf
Figure S1. Characterization of BdCV1-G1 RNA components, virion particles and structural proteins. (a) Agarose gel electrophoresis (1.0%) profile of dsRNA preparations extracted from mycelia of ZY7 and G1 after digestion with DNase I and S1 nuclease. Marker, Takara Wide Range DNA Marker (500-12,000 bp). (b) Agarose gel (1.0%) electrophoresis profile of BdCV1-G1 genomic dsRNA segments. dsRNAs were isolated from purified BdCV1-G1 virions (V-BdCV1-G1) and mycelia (M-BdCV1-G1). Marker, Takara Wide Range DNA Marker (500-12,000 bp). (c) Transmission electron micrograph of BdCV1-G1 virus particles from fungal strain G1. The particles were stained negatively with 2% uranyl acetate. (d) SDS-PAGE analysis of proteins purified from virion preparations from G1 mycelia (P-G1) and strain ZY7 (P-ZY7). Marker, protein molecular mass marker. * indicates the structural proteins of the virions. (e) Northern blot analysis of the BdCV1-G1 genomic dsRNAs. Probe sequences are listed in Table S1 (PDF 2001 kb)
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Figure S2. Multiple sequence alignments of the 5’- and 3’- terminal regions of the four dsRNAs of the BdCV1-G1 to BdCV1 consensus sequence (a) and phylogenetic analyses of RdRp of BdCV1-G1 and representative members of the families Chrysoviridae and Partitiviridae (b). Shading: black, 100% nucleotide sequence identity; gray, 60–80% nucleotide sequence identity. The BdCV1-conserved sequence in the 5’- and 3’- terminal regions is shown in the last line of the alignments, and star dots show identical nucleotides. The unrooted phylogenetic tree was constructed based on the neighbor-joining method using the program MEGA 5.0 with 1000 bootstrap replicates. The scale bar corresponded to the genetic distance (PDF 1107 kb)
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Figure S3. Pairwise alignment of the ORF1 deduced amino acid sequences of BdCV1-G1 and BdCV1. Black shading indicates identical amino acids (PDF 392 kb)
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Figure S4. Pairwise alignment of the ORF2 deduced amino acid sequences of BdCV1-G1 and BdCV1. Black shading indicates identical amino acids (PDF 367 kb)
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Ding, Z., Zhou, T. & Guo, LY. Characterization of a novel strain of Botryosphaeria dothidea chrysovirus 1 from the apple white rot pathogen Botryosphaeria dothidea . Arch Virol 162, 2097–2102 (2017). https://doi.org/10.1007/s00705-017-3320-6
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DOI: https://doi.org/10.1007/s00705-017-3320-6