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Complete genome sequence of rose virus A, the first carlavirus identified in rose

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Abstract

A novel virus was discovered in a Rosa wichuraiana Crep. by high-throughput sequencing and tentatively named “rose virus A” (RVA). Based on sequence identity and phylogenetic analysis, RVA represents a new member of the genus Carlavirus (family Betaflexiviridae). The genome of RVA is 8,849 nucleotides long excluding the poly(A) tail and contains six open reading frames (ORFs). The predicted ORFs code for a replicase, triple gene block (TGB), coat protein, and nucleic acid binding protein, as in a typical carlavirus. RVA is the first carlavirus identified in rose and has the highest nucleotide sequence similarity to poplar mosaic virus. Reverse transcription-PCR-based assays were developed to confirm the presence of RVA in the original source and to screen additional rose plants.

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Acknowledgements

Many thanks to Min Sook Hwang and Kristian Stevens (UC-Davis, Foundation Plant Services) for technical assistance.

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Authors and Affiliations

  1. Department of Plant Pathology, University of California-Davis, Davis, CA, 95616, USA

    Alfredo Diaz-Lara, Deborah Golino & Maher Al Rwahnih

  2. USDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA

    Dimitre Mollov

Authors
  1. Alfredo Diaz-Lara
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  2. Dimitre Mollov
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  3. Deborah Golino
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  4. Maher Al Rwahnih
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Correspondence to Maher Al Rwahnih.

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Handling Editor: Sead Sabanadzovic.

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Diaz-Lara, A., Mollov, D., Golino, D. et al. Complete genome sequence of rose virus A, the first carlavirus identified in rose. Arch Virol 165, 241–244 (2020). https://doi.org/10.1007/s00705-019-04460-1

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  • DOI: https://doi.org/10.1007/s00705-019-04460-1

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