Abstract
Bovine leukemia virus (BLV) infects cattle worldwide and causes B-cell lymphoma in cattle. BLV has been identified in human breast and lung cancer and in blood, but the association of BLV and human cancer is controversial. In this study, we investigated the existence of BLV in 145 Japanese human blood cell lines and 54 human cancer cell lines, using a new highly sensitive PCR assay that can amplify even one copy of BLV using LTR primers different from those in previous studies on BLV provirus in breast cancer. All samples were found negative for BLV provirus, suggesting that BLV is unlikely to infect humans.
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Acknowledgements
We thank Yoshinori Tanaka and Junko Nakamura for providing technical support, kind help, and suggestions. We also thank Tatsuro Saito for kindly providing the DNA from human cancer cell lines. We would like to thank Editage (http://www.editage.com) for English language editing. This work was supported by a Grant-in-Aid for Scientific Research (A) from the Japan Society for the Promotion of Science (JSPS) (Grant no. 16H02590) and by the Japan Society for the JSPS Postdoctoral Fellowship (Grant no. 18F18100).
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Human DNA samples derived from unrelated healthy volunteers were acquired from the Japanese Collection of Research Bioresources (JCRB) Cell Bank. The study protocol was approved by the Ethics Committee for the Protection of Human Subjects at RIKEN GENESIS CO., LTD.
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Saito, S., Kitamura-Muramatsu, Y., Komine, F. et al. Absence of bovine leukemia virus proviral DNA in Japanese human blood cell lines and human cancer cell lines. Arch Virol 165, 207–214 (2020). https://doi.org/10.1007/s00705-019-04474-9
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DOI: https://doi.org/10.1007/s00705-019-04474-9