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Complete genome sequence of Buttiauxella phage vB_ButM_GuL6

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Abstract

We present here the results of the analysis of the complete genome sequence of a lytic bacteriophage, vB_ButM_GuL6, which is the first virus isolated from Buttiauxella. Electron microscopy revealed that vB_ButM_GuL6 belongs to the family Myoviridae, order Caudovirales. The genome of vB_ButM_GuL6 is a linear, circularly permuted 178,039-bp dsDNA molecule with a GC content of 43.4%. It has been predicted to contain 282 protein-coding genes and two tRNA genes, tRNA-Met and tRNA-Gly. Using bioinformatics approaches, 99 (36%) of the vB_ButM_GuL6 genes were assigned a putative function. Genome-wide comparisons and phylogenetic analysis indicated that vB_ButM_GuL6 represents a new species of the subfamily Tevenvirinae and is most closely related to Escherichia virus RB43. These phages, together with Cronobacter phages Miller, CfP1, and IME-CF2, likely form a new genus within the subfamily Tevenvirinae.

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Data availability

The complete genome sequence of Buttiauxella phage vB_ButM_GuL6 was deposited in the GenBank database under the accession number MT334653.

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Funding

This study was supported by the Research Council of Lithuania (project no. SIT-7/2015).

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Authors and Affiliations

  1. Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University Life Sciences Center, Saulėtekio av. 7, 10257, Vilnius, Lithuania

    Algirdas Noreika, Rolandas Meškys & Laura Kaliniene

  2. Department of Eukaryote Gene Engineering, Institute of Biotechnology, Life Sciences Center, Saulėtekio av. 7, 10257, Vilnius, Lithuania

    Justas Lazutka

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  1. Algirdas Noreika
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  2. Rolandas Meškys
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  3. Justas Lazutka
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  4. Laura Kaliniene
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Correspondence to Laura Kaliniene.

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Noreika, A., Meškys, R., Lazutka, J. et al. Complete genome sequence of Buttiauxella phage vB_ButM_GuL6. Arch Virol 165, 2685–2687 (2020). https://doi.org/10.1007/s00705-020-04780-7

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  • DOI: https://doi.org/10.1007/s00705-020-04780-7

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