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The complete genomic sequence of the novel myovirus RP13 infecting Ralstonia solanacearum, the causative agent of bacterial wilt

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Abstract

A novel lytic bacteriophage, Ralstonia phage RP13, was isolated from tomato fields in Pang Nga, Thailand. Electron microscopic observation showed it to have the features of a myovirus with a novel triangulation number (T = 21, dextro). The RP13 DNA appeared to be heavily modified. By applying RNA sequencing and RNA-sequence-mediated DNA sequencing, the whole genome of RP31 was determined to be 170,942 bp in length with a mean G+C content of 39.2%. A total of 277 ORFs were identified as structural, functional, or hypothetical genes in addition to four tRNA genes. Phylogenetic analysis suggested that RP13 is not closely related to any other known phages. Thus, we concluded that the RP13 is a novel phage infecting R. solanacearum strains and will be a useful biocontrol agent against bacterial wilt disease.

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Acknowledgements

The authors are grateful to D. Yasuda and A. Bhunchoth for host range experiments.

Funding

This study was supported by the Strategic Japanese-Thai Research Cooperative Program (SICP) on Biotechnology (JST/BIOTEC-SICPTH2012) and the Japan Society for the Promotion of Science, KAKENHI (Grant number 15H04477).

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Author notes

  1. Takeru Kawasaki and Hisashi Endo contributed equally to this work.

Authors and Affiliations

  1. Unit of Biotechnology, Division of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-8530, Japan

    Takeru Kawasaki

  2. Bioinformatics Center, Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan

    Hisashi Endo & Hiroyuki Ogata

  3. Plant Research Laboratory, National Center for Genetic Engineering and Biotechnology, NSTDA, Pathum Thani, Thailand

    Orawan Chatchawankanphanich

  4. Hiroshima Study Center, The Open University of Japan, Hiroshima, 730-0053, Japan

    Takashi Yamada

Authors
  1. Takeru Kawasaki
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  2. Hisashi Endo
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  3. Hiroyuki Ogata
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  4. Orawan Chatchawankanphanich
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  5. Takashi Yamada
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Contributions

TY and TK conceived and designed the research. TK determined the RP13 genomic sequence. HE and HO were responsible for the genomic annotation and phylogenetic analyses. OC did experiments on phage isolation and characterization. TY and TK wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Takashi Yamada.

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The authors declare that they have no conflicts of interest.

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Kawasaki, T., Endo, H., Ogata, H. et al. The complete genomic sequence of the novel myovirus RP13 infecting Ralstonia solanacearum, the causative agent of bacterial wilt. Arch Virol 166, 651–654 (2021). https://doi.org/10.1007/s00705-020-04893-z

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  • DOI: https://doi.org/10.1007/s00705-020-04893-z

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