Abstract
Carnosine is present in high concentrations in skeletal muscle where it contributes to acid buffering and functions also as a natural protector against oxidative and carbonyl stress. Animal studies have shown an anti-diabetic effect of carnosine supplementation. High carnosinase activity, the carnosine degrading enzyme in serum, is a risk factor for diabetic complications in humans. The aim of the present study was to compare the muscle carnosine concentration in diabetic subjects to the level in non-diabetics. Type 1 and 2 diabetic patients and matched healthy controls (total n = 58) were included in the study. Muscle carnosine content was evaluated by proton magnetic resonance spectroscopy (3 Tesla) in soleus and gastrocnemius. Significantly lower carnosine content (−45%) in gastrocnemius muscle, but not in soleus, was shown in type 2 diabetic patients compared with controls. No differences were observed in type 1 diabetic patients. Type II diabetic patients display a reduced muscular carnosine content. A reduction in muscle carnosine concentration may be partially associated with defective mechanisms against oxidative, glycative and carbonyl stress in muscle.
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Abbreviations
- CN-1:
-
Carnosinase-1
- CNDP-1:
-
Carnosine dipeptidase-1 gene
- HNE:
-
4-Hydroxynonenal
- MRS:
-
Magnetic resonance spectroscopy
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Acknowledgments
This study was financially supported by grants from the Research Foundation—Flanders (FWO 1.5.149.08 and G.0046.09) and from Fundação de Amparo à Pesquisa do Estado de São Paulo (Grant numbers 2005/56464-9 and 2010/11221-0). The contribution of Johannes Ruige, Andries Pottier, Bert Celie, Melodie Arts, Koen De Meulenaer, Pieter Metsu, Vitor Painelli, and Rebeca Lugaresi is greatly acknowledged.
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The authors declare that there is no conflict of interest associated with this manuscript.
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Gualano, B., Everaert, I., Stegen, S. et al. Reduced muscle carnosine content in type 2, but not in type 1 diabetic patients. Amino Acids 43, 21–24 (2012). https://doi.org/10.1007/s00726-011-1165-y
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DOI: https://doi.org/10.1007/s00726-011-1165-y