Skip to main content
SpringerLink
Log in

HPTLC-based chromatographic studies in selected Justicia species for quality assessment

  • Original Research Paper
  • Published:
JPC – Journal of Planar Chromatography – Modern TLC Aims and scope Submit manuscript

Abstract

The genus Justicia (Acanthaceae) is a source of important pharmaceutical compounds. Justicia species are integral parts of several drug preparations in Indian indigenous healthcare system. However, J. beddomei and J. adhatoda are the authentic sources of ‘Vasa’. To authenticate assured therapeutic effect, proper identification at species level is critical. Phytochemical studies were carried out in different parts of seven Justicia species, viz., J. adhatoda, J. beddomei, J. betonica, J. carnea, J. gendarussa, J. santapaui and J. wynaadensis, using chromatographic analysis. Extracts of various parts like root, stem and leaf of the selected species were compared. The various parts showed remarkable differents in their chemical profiles. Comparative high-performance thin-layer chromatography (HPTLC) profile of seven Justicia species, various parts such as root, stem and leaf, have been developed and the same can be used as an analytical tool for the identification and authentication of genuine material and also as a ready reckoner to identify the authenticity of market raw drug samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13

Similar content being viewed by others

References

  1. Durkee LH (1986) Flora Costaricensis: Acanthaceae. Fieldiana Bot 18:1–87

    Google Scholar 

  2. Mabberley DJ (2008) Mabberley’s plant book: a portable dictionary of plants, their classification and uses, 3rd edn. Cambridge University Press, New York, NY, pp 1021

  3. Karthikeyan A, Shanti VM, Nagasathya A (2009) A preliminary phytochemical and antibacterial screening of crude extract of the leaf of Adhatoda vasica L. Int J Green Pharm 3:78–80

    Article  Google Scholar 

  4. Sunil CN, Prabhukumar KM, Naveen Kumar VV, Hareesh VS, Baiju EC (2015) A striking species of Justicia species L. (Acanthaceae) from Western Ghats of India. Kew Bull 70:1–6

    Article  Google Scholar 

  5. Corrêa GM, Alcântara AFDC (2012) Chemical constituents and biological activities of species of Justicia: a review. Rev Bras Farmacogn 22:220–238

    Article  Google Scholar 

  6. Chattopadhyay N, Nosál’ová G, Saha S, Bandyopadhyay SS, Flešková D, Ray B (2011) Structural features and antitussive activity of water extracted polysaccharide from Adhatoda vasica. Carbohydr Polym 83:1970–1974

    Article  CAS  Google Scholar 

  7. Subhashini S, Arunachalam KD (2011) Investigations on the phytochemical activities and wound healing properties of Adhatoda vasica leave in Swiss albino mice. Afr J Plant Sci 5:133–145

    Google Scholar 

  8. Suresh J, Muhameead RK, Hamath K, Nileena F, Rosemery K, Sinju PR, Nobin CJ (2019) Phytochemical screening of Justicia gendarussa. Int J Pharmacogn Chin Med 3:155

    Google Scholar 

  9. Sini S, Prashy P, George S (2018) Preliminary phytochemical investigation and estimation of polyphenolics in different parts of selected species of Justicia. Eur J Biomed Pharm Sci 5:460–464

    Google Scholar 

  10. Faiza R, Waqas KK, Adeel M, Muhammad G (2013) Detection of bioactive fractions of Justicia adhatoda leaves. Can J Appl Sci 1:388–398

    Google Scholar 

  11. Anonymous (2004) The ayurvedic pharmacopoeia of India (API). Part II, volume IV. Government of India, Ministry of Ayush. Pharmacopoeia Commission for Indian Medicine and Homeopathy, Ghaziabad

  12. Warrier PK, Nambiar VP, Ramankutty C (1993) Indian Medicinal Plants. III. Orient Longman Ltd., Chennai, pp 272–273

    Google Scholar 

  13. Prabhukumar KM, Beegam F, George S (2018) Revisiting the taxonomy of Justicia beddomei (Acanthaceae). Phytotaxa 350:71

    Article  Google Scholar 

  14. Gulfraz M, Arshad M, Nayyer N, Kanwal N, Nisar U (2004) Investigation of bioactive compounds of Berberis lycium Royle and Justicia adhatoda L. Ethanobot Leaflets 1:51–62

    Google Scholar 

  15. Anyakora C, Afolami I, EhianetaT Onwumere F (2008) HPLC analysis of nicotinamide, pyridoxine, riboflavin and thiamin in some selected food products in Nigeria. Afr J Pharm Pharmacol 2:029–036

    Google Scholar 

  16. Soni K, Naved T (2010) HPTLC: its applications in herbal drug industry. Pharmacol Rev 4:112–117

    Google Scholar 

  17. Sonal P, Maitreyi Z (2011) Pharmacognostic study of the root of Justicia gendarussa Burm. Asian J Trad Med 6:61–72

    Google Scholar 

  18. Suthar AC, Katkar KV, Patil PS, Hamarpurkar PD, Mridula G, Naik VR, Mundada GR, Chouhan VS (2009) Quantitative estimation of vasicine and vasicinone in Adhatoda vasica by HPTLC. J Pharm Res 2:1893–1899

    CAS  Google Scholar 

  19. Nikam PH, Kareparamban J, Jadhav A, Kadam V (2012) Future trends in standardization of herbal drugs. J Appl Pharm Sci 2:38–44

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, C.M.S. College, Kottayam, Kerala, India

    S. Sini

  2. Centre for Post Graduate Studies and Research in Botany, St. Joseph’s College (Autonomous), Devagiri, Calicut, Kerala, India

    Satheesh George

Authors
  1. S. Sini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Satheesh George
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Sini.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sini, S., George, S. HPTLC-based chromatographic studies in selected Justicia species for quality assessment. JPC-J Planar Chromat 33, 547–565 (2020). https://doi.org/10.1007/s00764-020-00076-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00764-020-00076-3

Keywords

Search

Navigation