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Antibacterial properties of copper iodide-doped glass ionomer-based materials and effect of copper iodide nanoparticles on collagen degradation

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Abstract

Objectives

This study investigated the antibacterial properties and micro-hardness of polyacrylic acid (PAA)-coated copper iodide (CuI) nanoparticles incorporated into glass ionomer-based materials, and the effect of PAA-CuI on collagen degradation.

Materials and methods

PAA-CuI nanoparticles were incorporated into glass ionomer (GI), Ionofil Molar AC, and resin-modified glass ionomer (RMGI), Vitrebond, at 0.263 wt%. The antibacterial properties against Streptococcus mutans (n = 6/group) and surface micro-hardness (n = 5/group) were evaluated. Twenty dentin beams were completely demineralized in 10 wt% phosphoric acid and equally divided in two groups (n = 10/group) for incubation in simulated body fluid (SBF) or SBF containing 1 mg/ml PAA-CuI. The amount of dry mass loss and hydroxyproline (HYP) released were quantified. Kruskal-Wallis, Student’s t test, two-way ANOVA, and Mann-Whitney were used to analyze the antibacterial, micro-hardness, dry mass, and HYP release data, respectively (p < 0.05).

Results

Addition of PAA-CuI nanoparticles into the glass ionomer matrix yielded significant reduction (99.999 %) in the concentration of bacteria relative to the control groups. While micro-hardness values of PAA-CuI-doped GI were no different from its control, PAA-CuI-doped RMGI demonstrated significantly higher values than its control. A significant decrease in dry mass weight was shown only for the control beams (10.53 %, p = 0.04). Significantly less HYP was released from beams incubated in PAA-CuI relative to the control beams (p < 0.001).

Conclusions

PAA-CuI nanoparticles are an effective additive to glass ionomer-based materials as they greatly enhance their antibacterial properties and reduce collagen degradation without an adverse effect on their mechanical properties.

Clinical relevance

The use of copper-doped glass ionomer-based materials under composite restorations may contribute to an increased longevity of adhesive restorations, because of their enhanced antibacterial properties and reduced collagen degradation.

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Acknowledgments

The authors wish to thank Mrs. Michelle Barnes for her outstanding secretarial support. The authors gratefully acknowledge the financial support from SC COBRE for Oral Health Research P20RR017696 and the Medical University of South Carolina Center for Oral Health Research. The authors declare no other conflicts.

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Authors and Affiliations

  1. Department of Oral Rehabilitation and Restorative Dentistry, College of Dental Medicine, Medical University of South Carolina, 173 Ashley Ave, Charleston, SC, 29425, USA

    Walter G. Renné & Anthony S. Mennito

  2. Department of Oral Health Sciences, College of Dental Medicine, Medical University of South Carolina, 173 Ashley Ave, Charleston, SC, 29425, USA

    Amanda Lindner, David Sentelle & Michael Defee

  3. Department of Oral Biology, College of Dental Medicine, Georgia Regents University, 1120 15th Street, Augusta, GA, 30912, USA

    Kelli A. Agee & David H. Pashley

  4. Highly Cited Investigator of King Abdulazis University School of Dentistry, Jeddah, Saudi Arabia

    David H. Pashley

  5. Department of Chemistry, Clemson University, 116 Biosystems Research Complex, Clemson, SC, 29634, USA

    Daniel Willett

  6. Department of Microbiology and Immunology, Medical University of South Carolina, 173 Ashley Ave, MSC504, Charleston, SC, 29425-0504, USA

    Michael Schmidt

  7. Department of Restorative Dentistry, School of Dental Medicine, State University of New York at Buffalo, 3435 Main Street, Buffalo, NY, 14214, USA

    Camila Sabatini

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  1. Walter G. Renné
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  2. Amanda Lindner
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  8. Michael Defee
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  10. Camila Sabatini
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Correspondence to Camila Sabatini.

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Funding

This study was funded by SC COBRE for Oral Health Research; award number: P20RR017696 to Dr. Walter G. Renne.

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Renné, W.G., Lindner, A., Mennito, A.S. et al. Antibacterial properties of copper iodide-doped glass ionomer-based materials and effect of copper iodide nanoparticles on collagen degradation. Clin Oral Invest 21, 369–379 (2017). https://doi.org/10.1007/s00784-016-1799-y

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