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Salivary oxidative stress biomarkers in chronic periodontitis and acute coronary syndrome

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Abstract

Objectives

The study aimed at assessing oxidative stress (OS) biomarker levels in the saliva of patients with chronic periodontitis (CP) and acute coronary syndrome (ACS) and establishing their correlation to periodontal parameters and markers for cardiovascular events.

Materials and methods

The present study enrolled 24 patients with ACS and CP (the ACSCP group), 24 patients with ACS only (the ACS group), 24 patients with CP only (the CP group), and 24 healthy controls. Plaque index (PI), gingival index, bleeding on probing, probing pocket depth (PPD), and clinical attachment loss were recorded. Markers for cardiovascular events included serum high sensitivity C-reactive protein (hsCRP) and plasma fibrinogen. 8-Hydroxydeoxyguanosine (8-OHdG), protein carbonyl (PC), malondialdehyde (MDA), and total antioxidant capacity (TAOC) were used as OS biomarkers.

Results

Salivary 8-OHdG, MDA, and PC levels were significantly higher in the ACSCP, ACS, and CP groups than in healthy controls (p < 0.05). There were significant correlations between salivary PC levels and PI or PPD (p < 0.05) as well as between salivary 8-OHdG levels and all periodontal parameters (p < 0.05). TAOC levels in saliva were correlated to both serum hsCRP and plasma fibrinogen (p < 0.05). Salivary MDA levels were correlated to all periodontal parameters and biomarkers for cardiovascular events (p < 0.05).

Conclusions

Salivary OS biomarker levels were higher in diseased groups compared to control. They also correlated to clinical periodontal parameters and markers for cardiovascular events in ACS patients, with or without CP.

Clinical relevance

Salivary OS biomarkers could potentially serve as diagnostic tools for cardiovascular and/or periodontal diseases.

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Acknowledgements

This study was supported by the International Dental Collaboration of the Mekong River Region (IDCMR) scholarship from the Faculty of Dentistry, Mahidol University, Bangkok, Thailand, and by the Office of the Higher Education Commission and Mahidol University under the National Research Universities Initiative, Thailand. The authors would like to thank Dr. Dung Ngoc Kieu of the Department of Cardiology; Ms. Giao Quynh Thi Phung of the Department of Biochemistry, Cho Ray Hospital, Ho Chi Minh City, Vietnam; and Ms. Duangchewan Puengsurin of the Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand, for their contribution to this study.

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Authors and Affiliations

  1. Department of Oral Biology, Faculty of Dentistry, Mahidol University, 6 Yothi Street, Ratchathewi, Bangkok, 10400, Thailand

    Thuy T. Nguyen & Rudee Surarit

  2. Department of Periodontology, Faculty of Odonto-Stomatology, University of Medicine and Pharmacy—Ho Chi Minh City, Ho Chi Minh City, Vietnam

    Thuy T. Nguyen

  3. Department of Dental Basic Sciences, Faculty of Odonto-Stomatology, University of Medicine and Pharmacy—Ho Chi Minh City, Ho Chi Minh City, Vietnam

    Lan Q. Ngo

  4. Department of Oral Medicine and Periodontology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand

    Ananya Promsudthi

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  1. Thuy T. Nguyen
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  4. Rudee Surarit
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Correspondence to Rudee Surarit.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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The authors declare that they have no conflicts of interest.

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Informed consent was obtained from all individual participants included in the study.

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Nguyen, T.T., Ngo, L.Q., Promsudthi, A. et al. Salivary oxidative stress biomarkers in chronic periodontitis and acute coronary syndrome. Clin Oral Invest 21, 2345–2353 (2017). https://doi.org/10.1007/s00784-016-2029-3

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  • DOI: https://doi.org/10.1007/s00784-016-2029-3

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