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Saccharopolyspora lacisalsi sp. nov., a novel halophilic actinomycete isolated from a salt lake in Xinjiang, China

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Abstract

A novel actinomycete strain, designated TRM 40133T, was isolated from a hypersaline habitat of Tarim basin in Xinjiang Province, north-west China. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of the strain showed that it formed a well-seperated sub-branch within the radiation of the genus Saccharopolyspora. The highest levels of 16S rRNA gene sequence similarity was found between the strain TRM 40133T and Saccharopolyspora qijiaojingensis YIM 91168T (96.5%). The chemotaxonomic characteristics of the isolate are typical for the genus Saccharopolyspora. It contained meso-DAP as the diagnostic diamino acid. Whole cell hydrolysate contained arabinose, xylose, ribose and glucose. The diagnostic phospholipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and two unknown phospholipids. The main menaquinone was MK-9(H6) and MK-9(H4). No mycolic acid was detected. The predominant cellular fatty acids were iso-C16:0 and anteiso-C17:0. The G+C content of the genomic DNA was 68.2 mol%. In addition, the strain TRM 40133T had a phenotypic profile that readily distinguished it from the recognized representatives of the genus Saccharopolyspora. The strain TRM 40133T therefore represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora lacisalsi sp. nov. is proposed. The type strain is TRM 40133T (=KCTC 19987T =CCTCC AA 2010012T).

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Acknowledgments

This research was supported by National Natural Science Foundation of China (Project no. 31060003, 31060001), Opening Project by State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (Project no. SKLMR-20090603), Key Project for Department of Science and Technology of Ministry of Education of China (Project no. 209145), 973 Program (Project no. 2010CB134505) and the Opening Project of Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography (Project no. LBB-2010-004).

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Authors and Affiliations

  1. Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, 843300, Xinjiang, People’s Republic of China

    Tong-Wei Guan & Li-Li Zhang

  2. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Tong-Wei Guan, Zhan-Feng Xia, Ji-Sheng Ruan & Ying Huang

  3. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People’s Republic of China

    Nan Wu

  4. Faculty of Resource and Environmental Sciences, Sichuan Agricultural University, Yaan, 625000, People’s Republic of China

    Xiao-Ping Zhang

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  1. Tong-Wei Guan
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Correspondence to Tong-Wei Guan, Ying Huang or Li-Li Zhang.

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Communicated by A. Oren.

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Guan, TW., Wu, N., Xia, ZF. et al. Saccharopolyspora lacisalsi sp. nov., a novel halophilic actinomycete isolated from a salt lake in Xinjiang, China. Extremophiles 15, 373–378 (2011). https://doi.org/10.1007/s00792-011-0369-0

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