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Evaluation of the effects of different supplementation on oxidative status in patients with rheumatoid arthritis

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Abstract

Rheumatoid arthritis is a chronic inflammatory disease. Reactive oxygen species have been considered as aggravating factors for autoimmune diseases. Fatty acids had been linked in reduction of various diseases by augment of their antioxidant potential and antiinflammatory mechanisms. The aim of this study was to assess the oxidative status in patients with rheumatoid arthritis who used concentrated fish oil only or concentrated fish oil in combination with evening primrose oil in a period of 3 months. Subjects were divided into three groups. The group I consists of patients who had been taking only their regular rheumatologic therapy; group II, patients who had been taking concentrated fish oil; and group III, patients who had been taking concentrated fish oil and evening primrose oil. Peripheral blood samples were used for all the assays. We assessed the following oxidative stress markers: index of lipid peroxidation (thiobarbituric acid-reactive substances (TBARS)), hydrogen peroxide (H2O2), superoxide anion radical (O2 −), nitric oxide (NO), superoxide dismutase activity (SOD), catalase activity (CAT), and glutathione levels (GSH) in erythrocytes. There were no statistically significant changes for any of the oxidative stress parameters in group I. In group II, levels of TBARS, NO2 −, and GSH were increased, while levels of H2O2 decreased. Increased values of TBARS, NO2 −, and SOD were found in group III. Our findings indicate that intakes of fish oil and evening primrose oil may be of importance in mitigation of inflammation, disease activity, and oxidative stress biomarkers, through increased activities of antioxidant enzymes.

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Acknowledgments

This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 175043), and the Faculty of Medical Sciences, University of Kragujevac (Junior Project 01/14).

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Authors and Affiliations

  1. Department of Hygiene, Institute for Public Health, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

    Dragan Vasiljevic

  2. Department of Rheumatology, Internal Clinic, Clinical Center Kragujevac, Kragujevac, Serbia

    Mirjana Veselinovic & Aleksandra Lucic-Tomic

  3. Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

    Maja Jovanovic & Nevena Jeremic

  4. Centre of Research Excellence in Nutrition and Metabolism, Institute of Medical Research, University of Belgrade, Belgrade, Serbia

    Aleksandra Arsic & Vesna Vucic

  5. Department of Medical Sciences, Faculty for Tourism, University of Kragujevac, Vrnjacka Banja, Serbia

    Sandra Zivanovic

  6. Institute of Medical Physiology “Richard Burian,” Faculty of Medicine, University of Belgrade, Belgrade, Serbia

    Dragan Djuric

  7. Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 69 Svetozara Markovica, 34000, Kragujevac, Serbia

    Vladimir Jakovljevic

Authors
  1. Dragan Vasiljevic
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  2. Mirjana Veselinovic
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  3. Maja Jovanovic
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  4. Nevena Jeremic
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  5. Aleksandra Arsic
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  6. Vesna Vucic
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  7. Aleksandra Lucic-Tomic
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  8. Sandra Zivanovic
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Correspondence to Vladimir Jakovljevic.

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Vasiljevic, D., Veselinovic, M., Jovanovic, M. et al. Evaluation of the effects of different supplementation on oxidative status in patients with rheumatoid arthritis. Clin Rheumatol 35, 1909–1915 (2016). https://doi.org/10.1007/s10067-016-3168-2

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  • DOI: https://doi.org/10.1007/s10067-016-3168-2

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