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Anti-adipogenic effect of Lactobacillus fermentum MG4231 and MG4244 through AMPK pathway in 3T3-L1 preadipocytes

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Abstract

This study evaluated the anti-adipogenic effects and mechanisms underlying the action of Lactobacillus fermentum MG4231 and MG4244 strains on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes. Treatment with cell-free extracts (CFEs) from the two strains reduced lipid accumulation and intracellular triglyceride production in 3T3-L1 adipocytes by more than 50%. The inhibitory effects of L. fermentum on lipid accumulation were mediated by the downregulation of FAS and aP2 resulting from the inhibition of PPARγ and C/EBPα gene expression. Moreover, AMPK and HSL phosphorylation was upregulated by CFE treatment. These results indicated that the anti-adipogenic and lipolysis activities of L. fermentum strains were caused by increased AMPK and HSL phosphorylation. Both strains displayed high leucine arylamidase and β-galactosidase enzymatic activity, with excellent adhesion to epithelial cells. Therefore, we identified L. fermentum as potential new probiotics for the prevention of obesity.

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Abbreviations

CFE:

Cell-free extract

DEX:

Dexamethasone

IBMX:

Isobutylmethylxanthine

DMEM:

Dulbecco modified Eagle’s medium

FBS:

Fetal bovine serum

TG:

Triglyceride

MTT:

Tetrazolium bromide salt

PPARγ:

Peroxisome proliferator-activated receptor γ

C/EBPα:

CCAAT/enhancer-binding protein α

FAS:

Fatty acid synthase

aP2:

Adipose-specific fatty acid-binding protein

SREBP1:

Sterol regulatory element-binding protein 1

LPL:

Lipoprotein lipase

ACC:

Acetyl CoA carboxylase

AMPK:

AMP-activated protein kinase

p-AMPK:

Phospho-AMP-activated protein kinase

HSL:

Hormone-sensitive lipase

p-HSL:

Phospho-hormone- sensitive lipase

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Acknowledgements

This work was supported by a project for Collabo R&D between Industry, Academy, and Research Institute funded by the Korea Ministry of SMEs and Startups in 2019 (Project No. S2717946).

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Authors and Affiliations

  1. Department of Bio-Health Convergence Major, Duksung Women’s University, Seoul, 01369, Republic of Korea

    SukJin Kim, Soo-Im Choi & Gun-Hee Kim

  2. Department of Food and Nutrition, Duksung Women’s University, Seoul, 01369, Republic of Korea

    Gun-Hee Kim

  3. Department of Food Science, Purdue University, West Lafayette, IN, 47906, USA

    Miran Jang

  4. MEDIOGEN, Co., Ltd., Jecheon, 27159, Republic of Korea

    Yulah Jeong & Chang-Ho Kang

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  1. SukJin Kim
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Kim, S., Choi, SI., Jang, M. et al. Anti-adipogenic effect of Lactobacillus fermentum MG4231 and MG4244 through AMPK pathway in 3T3-L1 preadipocytes. Food Sci Biotechnol 29, 1541–1551 (2020). https://doi.org/10.1007/s10068-020-00819-2

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