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Photobiomodulation effects on mRNA levels from genomic and chromosome stabilization genes in injured muscle

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Abstract

Muscle injuries are the most prevalent type of injury in sports. A great number of athletes have relapsed in muscle injuries not being treated properly. Photobiomodulation therapy is an inexpensive and safe technique with many benefits in muscle injury treatment. However, little has been explored about the infrared laser effects on DNA and telomeres in muscle injuries. Thus, the aim of this study was to evaluate photobiomodulation effects on mRNA relative levels from genes related to telomere and genomic stabilization in injured muscle. Wistar male rats were randomly divided into six groups: control, laser 25 mW, laser 75 mW, injury, injury laser 25 mW, and injury laser 75 mW. Photobiomodulation was performed with 904 nm, 3 J/cm2 at 25 or 75 mW. Cryoinjury was induced by two applications of a metal probe cooled in liquid nitrogen directly on the tibialis anterior muscle. After euthanasia, skeletal muscle samples were withdrawn and total RNA extracted for evaluation of mRNA levels from genomic (ATM and p53) and chromosome stabilization (TRF1 and TRF2) genes by real-time quantitative polymerization chain reaction. Data show that photobiomodulation reduces the mRNA levels from ATM and p53, as well reduces mRNA levels from TRF1 and TRF2 at 25 and 75 mW in injured skeletal muscle. In conclusion, photobiomodulation alters mRNA relative levels from genes related to genomic and telomere stabilization in injured skeletal muscle.

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Role of funding source

This work was supported by Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Conselho Nacional de Pesquisa (CNPq).

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Authors and Affiliations

  1. Laboratório de Pesquisa em Células Tronco, Departamento de Histologia e Embriologia, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, Vila Isabel, Rio de Janeiro, 20551-030, Brazil

    Larissa Alexsandra da Silva Neto Trajano & Ana Carolina Stumbo

  2. Laboratório de Biomorfologia e Patologia Experimental, Universidade Severino Sombra, Avenida Expedicionário Oswaldo de Almeida Ramos 280, Vassouras, Rio de Janeiro, 27700-000, Brazil

    Larissa Alexsandra da Silva Neto Trajano & Eduardo Tavares Lima Trajano

  3. Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, Vila Isabel, Rio de Janeiro, 20551-030, Brazil

    Luiz Philippe da Silva Sergio, Adilson Fonseca Teixeira, Andre Luiz Mencalha & Adenilson de Souza da Fonseca

  4. Departamento de Ciências Fisiológicas, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Rio de Janeiro, 20211-040, Brazil

    Adenilson de Souza da Fonseca

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  1. Larissa Alexsandra da Silva Neto Trajano
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  3. Luiz Philippe da Silva Sergio
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Correspondence to Adenilson de Souza da Fonseca.

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This study received Universidade do Estado do Rio de Janeiro Research Ethics Committee’s approval, process number CEUA/024/2014.

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da Silva Neto Trajano, L.A., Trajano, E.T.L., da Silva Sergio, L.P. et al. Photobiomodulation effects on mRNA levels from genomic and chromosome stabilization genes in injured muscle. Lasers Med Sci 33, 1513–1519 (2018). https://doi.org/10.1007/s10103-018-2510-0

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  • DOI: https://doi.org/10.1007/s10103-018-2510-0

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