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Elucidation of Gut Microbiota in Mud Crab Scylla paramamosain Challenged to WSSV and Aeromonas hydrophila

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Abstract

Mud crab Scylla paramamosain (S. paramamosain) is an economically important marine crab species around the world. White spot syndrome virus (WSSV) and Aeromonas hydrophila (AH) are pathogens during mud crab mariculture. It has been reported that gut microbiota possessed a great impact on the host development, nutrition, immunity, and disease resistance. However, little information was known about the impacts of WSSV or AH infection on the structure, composition, and function of the gut microbiotain of mud crabs. In this study, the gut microbiota of mud crabs infected with A. hydrophila and WSSV were characterized. The results showed that the composition and bacteria correlation of the gut microbiota were significantly decreased. During A. hydrophila infection, the pathogens played a major regulatory role in host. While in the mud crabs infected with WSSV, many beneficial strains had a great impact on the host expect for the pathogens. Therefore, our study revealed the effect of pathogens infection on gut microbiota of mud crabs and clarified the difference between viral infection and bacterial infection.

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Funding

This study was financially supported by the National Natural Science Foundation of China (31802341), Natural Science Foundation of Guangdong Province, China, (2018A030307044), Department of Education of Guangdong Province, China, (2017KQNCX072), and STU Scientific Research Foundation for Talents (NTF18001).

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Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, 515063, China

    Shanmeng Lin, Tongtong Kong, Xin Ren, Shengkang Li & Yi Gong

  2. Institute of Marine Sciences, Shantou University, Shantou, 515063, China

    Shanmeng Lin, Tongtong Kong, Xin Ren, Shengkang Li & Yi Gong

  3. STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China

    Shanmeng Lin, Tongtong Kong, Xin Ren, Shengkang Li & Yi Gong

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  1. Shanmeng Lin
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  3. Xin Ren
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Contributions

SML and TTK performed the experiments and analyzed the data. SML and YG wrote the manuscript. All authors read and approved the contents of the manuscript and its publication.

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Correspondence to Yi Gong.

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Highlights

• The gut microbiota in mud crab during A. hydrophila and WSSV infection were characterized.

• The composition and bacteria correlation of the gut microbiota significantly decreased during the infections.

• During A. hydrophila infection, the pathogenic bacteria play the major role in host.

• During WSSV infection, many beneficial strains possess great impacts on the host expect for the pathogenic bacteria.

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Lin, S., Kong, T., Ren, X. et al. Elucidation of Gut Microbiota in Mud Crab Scylla paramamosain Challenged to WSSV and Aeromonas hydrophila. Mar Biotechnol 22, 661–672 (2020). https://doi.org/10.1007/s10126-020-09987-y

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