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A Novel Dop2/Invertebrate-Type Dopamine Signaling System Potentially Mediates Stress, Female Reproduction, and Early Development in the Pacific Oyster (Crassostrea gigas)

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Abstract

The dopaminergic signaling pathway is involved in many physiological functions in vertebrates, but poorly documented in protostome species except arthropods. We functionally characterized a novel dopamine receptor in the Pacific oyster (Crassostrea gigas), activated by dopamine and tyramine with different efficacy and potency orders. This receptor — Cragi-DOP2R — belongs to the D1-like family of receptors and corresponds to the first representative of the Dop2/invertebrate-type dopamine receptor (Dop2/INDR) group ever identified in Lophotrochozoa. Cragi-DOP2R transcripts were expressed in various adult tissues, with higher expression levels in the visceral ganglia and the gills. Following an experiment under acute osmotic conditions, Cragi-DOP2R transcripts significantly increased in the visceral ganglia and decreased in the gills, suggesting a role of dopamine signaling in the mediation of osmotic stress. Furthermore, a role of the Cragi-DOP2R signaling pathway in female gametogenesis and in early oyster development was strongly suggested by the significantly higher levels of receptor transcripts in mature female gonads and in the early embryonic stages.

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Acknowledgements

We are grateful to Marie-Pierre Dubos for her technical assistance.

Funding

This work was funded by the ANR project NEMO (Agence Nationale de la Recherche 14CE02 0020). J.S.’s PhD fellowship was co-financed by the NEMO project and by the European Union within the framework of the operational program FEDER/FSE 2014–2020.

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  1. UMR BOREA, Normandie Université, UNICAEN, Sorbonne Universités, IRD-207, Esplanade de la Paix, CNRS-806714032, CAEN cedex 5, MNHN, France

    Julie Schwartz, Emilie Réalis-Doyelle, Lorane Le Franc & Pascal Favrel

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  2. Emilie Réalis-Doyelle
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  3. Lorane Le Franc
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Conceptualization: P.F. Methodology: J.S, E.R-D, P.F. Software: J.S, L.LF. Validation: J.S, P.F. Formal analysis: J.S, L.LF, E.R-D, P.F. Investigation: J.S, P.F. Writing — original draft: J.S, P.F. Writing — review and editing: J.S, P.F. Supervision, P.F. Project administration, P.F. Funding acquisition: P.F.

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Schwartz, J., Réalis-Doyelle, E., Le Franc, L. et al. A Novel Dop2/Invertebrate-Type Dopamine Signaling System Potentially Mediates Stress, Female Reproduction, and Early Development in the Pacific Oyster (Crassostrea gigas). Mar Biotechnol 23, 683–694 (2021). https://doi.org/10.1007/s10126-021-10052-5

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