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Association of serum lncRNA H19 expression with inflammatory and oxidative stress markers and routine biochemical parameters in chronic kidney disease

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Abstract

Background

Chronic kidney disease (CKD) is a disorder that affects millions worldwide, and current treatment options aiming at inhibiting the progression of kidney damage are limited. Long noncoding RNA (lncRNA) H19 is one of the first explored lncRNAs and its deregulation is associated with renal pathologies, such as renal cell injury and nephrotic syndrome. However, there is still no research investigating the connection between serum lncRNA H19 expressions and clinical outcomes in CKD patients. Therefore, we investigated the relation of serum lncRNA H19 expressions with routine biochemical parameters, inflammatory cytokines, oxidative stress and mineralization markers in advanced CKD patients.

Methods

lncRNA H19 serum levels from 56 CKD patients and 20 healthy controls were analyzed with reverse-transcription quantitative polymerase chain reaction method. Serum tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), and osteocalcin (OC) levels were measured with enzyme linked-immunosorbent assay. Total antioxidant status (TAS) and total oxidative status (TOS) levels were evaluated by the routine measurement method.

Results

We found that lncRNA H19 expressions were upregulated in patients with CKD compared to the controls. Furthermore, lncRNA H19 relative expression levels showed a negative relationship with glomerular filtration rate (GFR) while it was positively correlated with ferritin, phosphorus, parathyroid hormone, TNF-α, IL-6, OC, TAS and TOS levels.

Conclusion

lncRNA H19 expressions were increased in CKD stage 3–5 and HD patients, and elevated lncRNA H19 expressions were associated with decreased glomerular filtration rate, inflammation, and mineralization markers in these patients.

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Funding

This work is supported by the Scientific Research Project Fund of Hatay Mustafa Kemal University under project number: 19.M.013.

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Authors and Affiliations

  1. Department of Physiotherapy and Rehabilitation, Faculty of Health Sciences, Sakarya University of Applied Sciences, Sakarya, Turkey

    Hamza Malik Okuyan

  2. Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada

    Hamza Malik Okuyan

  3. Department of Biochemistry, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey

    Serdar Dogan

  4. Department of Medical Biology, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey

    Menderes Yusuf Terzi

  5. Department of Molecular Biochemistry and Genetics, Graduate School of Health Sciences, Hatay Mustafa Kemal University, Hatay, Turkey

    Menderes Yusuf Terzi

  6. Department of Epidemiology and Biostatistics, Schulich School of Medicine and Dentistry, Ivey Business School, University of Western Ontario, London, Ontario, Canada

    Mehmet A. Begen

  7. Department of Nephrology, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey

    Faruk Hilmi Turgut

Authors
  1. Hamza Malik Okuyan
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  2. Serdar Dogan
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  3. Menderes Yusuf Terzi
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  4. Mehmet A. Begen
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  5. Faruk Hilmi Turgut
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Contributions

HMO, SD and FHT designed the study. HMO, SD, and MYT performed genetic and biochemistry laboratory analyses. HMO, SD, MYT, MAB, and FHT wrote/drafted/edited the manuscript and interpreted the results. HMO, SD, MYT, MAB, and FHT conducted analyses, prepared graphs/figures and revised the manuscript.

Corresponding author

Correspondence to Hamza Malik Okuyan.

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Conflict of interest

We all declare that we do not have any conflicts of interest.

Ethical approval

All procedures performed in the present study were approved by the institutional review board and were in compliance with the 1964 Helsinki Declaration (Approval number: 2019/26).

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Okuyan, H.M., Dogan, S., Terzi, M.Y. et al. Association of serum lncRNA H19 expression with inflammatory and oxidative stress markers and routine biochemical parameters in chronic kidney disease. Clin Exp Nephrol 25, 522–530 (2021). https://doi.org/10.1007/s10157-021-02023-w

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  • DOI: https://doi.org/10.1007/s10157-021-02023-w

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