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Phylogenetic relationships of Petrocoptis A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula

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Abstract

Petrocoptis is a small genus of chasmophytic plants endemic to the Iberian Peninsula, with some localized populations in the French Pyrenees. Within the genus, a dozen species have been recognized based on morphological diversity, most of them with limited distribution area, in small populations and frequently with potential threats to their survival. To date, however, a molecular evaluation of the current systematic treatments has not been carried out. The aim of the present study is to infer phylogenetic relationships among its subordinate taxa by using plastidial rps16 intron and nuclear internal transcribed spacer (ITS) DNA sequences; and evaluate the phylogenetic placement of the genus Petrocoptis within the family Caryophyllaceae. The monophyly of Petrocoptis is supported by both ITS and rps16 intron sequence analyses. Furthermore, time estimates using BEAST analyses indicate a Middle to Late Miocene diversification (10.59 Myr, 6.44–15.26 Myr highest posterior densities [HPD], for ITS; 14.30 Myr, 8.61–21.00 Myr HPD, for rps16 intron).

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Acknowledgments

The authors thank René Pérez for laboratory assistance. We would like to thank Álvaro Bueno, Candela Cuesta, Daniel Goñi, Francisco Javier Fernández Casas, Itziar García, Javier Loidi, José Luis Benito, Juan Antonio Campos, Mercedes Herrera and Miren Idoia Biurrun for their help and critical reading of the manuscript.

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Authors and Affiliations

  1. Departamento de Biología de Organismos y Sistemas, Área de Botánica, Universidad de Oviedo, Catedrático Rodrigo Uría s/n, 33071, Oviedo, Spain

    Eduardo Cires & José Antonio Fernández Prieto

  2. Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400, Klosterneuburg, Austria

    Eduardo Cires

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  1. Eduardo Cires
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  2. José Antonio Fernández Prieto
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Correspondence to Eduardo Cires.

Appendices

Appendix 1

See Table 3.

Table 3 Genera and type species for the family Caryophyllaceae used to estimate divergence times of Petrocoptis
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Appendix 2

See Table 4.

Table 4 Estimated divergence times of Petrocoptis and related lineages from two different data sets (ITS, rps16) implemented in BEAST. A calibration time of 34 million years (Myr) for the split between subfamilies Alsinoideae and Caryophylloideae was used
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Cires, E., Prieto, J.A.F. Phylogenetic relationships of Petrocoptis A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula. J Plant Res 128, 223–238 (2015). https://doi.org/10.1007/s10265-014-0691-6

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