Skip to main content

Advertisement

SpringerLink
Log in

Reduced abscisic acid content is responsible for enhanced sucrose accumulation by potassium nutrition in vegetable soybean seeds

  • Regular Paper
  • Published:
Journal of Plant Research Aims and scope Submit manuscript

Abstract

In order to understand the physiological mechanism of potassium (K) application in enhancing sugar content of vegetable soybean seeds, pot experiments were conducted in 2014 and 2015 with two vegetable soybean (Glycine max L. Merr.) cultivars (c.v. Zhongkemaodou 1 and c.v. 121) under normal rate of nitrogen and phosphorus application. Three potassium (K) fertilization treatments were imposed: No K application (K0), 120 kg K2SO4 ha−1 at seeding (K1), and 120 kg K2SO4 ha−1 at seedling + 1% K2SO4 foliar application at flowering (K2). Contents of indole-3-acetic acid (IAA), gibberellins (GA), cytokinins (ZR) and abscisic acid (ABA) in seeds were determined from 4 to 8 weeks after flowering. K fertilization increased the contents of IAA, GA, ZR, soluble sugar, sucrose and fresh pod yield, but reduced ABA content consistently. When the contents of soluble sugar and sucrose reached the highest level at 7 weeks after flowering for the 2 cultivars, the contents of IAA、GA、ZR all reached the lowest level in general. The content of ABA in seed was negatively correlated with the sucrose content (P < 0.01, r = −0.749**, −0.768** in 2014 and −0.535**, −0.791** in 2015 for c.v.121 and c.v. Zhongkemaodou 1 respectively). The changes in ratio of the ABA to (IAA + GA + ZR) from 4 to 8 weeks after flowering affected by K application were coincident to the changes of sucrose accumulation. The reduced ratio of ABA/(IAA + GA + ZR) affected by K nutrition particularly reduced abscisic acid content plays a critical role in enhancing sucrose content, which might be a partial mechanism involved in K nutrition to improve the quality of vegetable soybean.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Bauer H, Ache P, Lautner S, Fromm J, Hartung W, Al-Rasheid KA, Mendel RR (2013) The stomatal response to reduced relative humidity requires guard cell-autonomous ABA synthesis. Curr Biol 23:53–57

    Article  CAS  PubMed  Google Scholar 

  • Bhatia S, Singh R (2002) Phytohormone-mediated transformation of sugars to starch in relation to the activities of amylases, sucrose-metabolising enzymes in sorghum grain. Plant Growth Regul 36:97–104

    Article  CAS  Google Scholar 

  • Cakmak I (2005) The role of potassium in alleviating detrimental effects of abiotic stresses in plants. J Plant Nutr Soil Sci 168:521–530

    Article  CAS  Google Scholar 

  • Cakmak I, Hengeler C, Marschner H (1994) Changes in phloem export of sucrose in leaves in response to phosphorus, potassium and magnesium deficiency in bean plants. J Exp Bot 45:1251–1257

    Article  CAS  Google Scholar 

  • Cao Y, Zhao H (2009) Effect of potassium application on forming and accumulation of sucrose in sweet maize. J Northeast Agric Univ 7:008 (Chinese with english summary)

    Google Scholar 

  • Chen L, Zhu YL, Yang LF, Wei GP, Wang G (2010) Effects of nitrogen forms and ratios on carbon-nitrogen assimilation in developing seeds of vegetable soybean. Acta Bot Boreali-Occident Sin 30:323–329

  • Cheng Y, Diao F, Wu N, Huang M (1998) Effects of sucrose regulation culture on endogenous ABA levels of carrot somatic embryo. Acta Bot Sin 41:761–765

  • Davies PJ (2004) Plant hormones: biosynthesis, signal transduction, action!. Springer Science and Business Media

  • Davies P (2012) Plant hormones and their role in plant growth and development. Springer Science and Business Media

  • Davies WJ, Jones HG (1991) Abscisic acid physiology and biochemistry. BIOS Scientific Publishers

  • Dong ZX, Fu JM, Zhang YX (2001) Hormone regulating effect for cultivation physiology on soybean. J Shihezi Univ 5:339–341 (Chinese with english summary)

    CAS  Google Scholar 

  • Du M, Li YS, Zhang QY, Gu SY, Liu XB (2012) Effects of potassium fertilizer on chlorophyll content of vegetable soybean in reproductive stages. Soybean Sci 31:941–946

  • Fehr WR, Caviness CE, Burmood DT, Pennington JS (1971) Stage of development descriptions for soybeans, Glycine max (L.) Merrill. Crop Sci 11:929–931

    Article  Google Scholar 

  • Guo HX, Da PZ, Wen SJ (2000) Effects of IAA, GA and ABA on 14 C-sucrose import and metabolism in grape berries. Acta Hortic Sin 27:6–10

  • Guo Y, Song XL, Wang QC, Li YJ, Sun XZ (2006) Effect of potassium nutrition on endogenous hormone and free radicals in cotton seedling. Acta Agric Boreali-Sinic 1:013

  • Huber SC, Israel DW (1982) Biochemical basis for partitioning of photosynthetically fixed carbon between starch and sucrose in soybean (glycine-max-merr) leaves. Plant physiol 69:691–696

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Iglesias DJ, Tadeo FR, Legaz F, Primo-Millo E, Talon M (2001) In vivo sucrose stimulation of colour change in citrus fruit epicarps: interactions between nutritional and hormonal signals. Physiol Plant 112:244–250

  • Jaillais Y, Chory J (2010) Unraveling the paradoxes of plant hormone signaling integration. Nat Struct Mol Biol 17:642–645

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ku WZ, Deng KQ, Zhang XQ, Tong JH, Zhou H, Xiao LT (2009) Effects of low potassium stress on mineral nutrient absorption and phytohormone contents of rice seedling. Plant Nutri Ferti Sci 15:69–75

  • Kurapov PB, Skorobogatova IV, Sal’nikova EI, Sorkina GL, Siusheva AG (1999) Diurnal cycles of endogenous phytohormones in barley. Izvestiia Akademii nauk. Seriia biologicheskaia/Rossiiskaia akademiia nauk 1:108–114

  • Liu L, Cang J, Yu J, Wang X, Huang R, Wang J, Lu B (2013) Effects of exogenous abscisic acid on carbohydrate metabolism and the expression levels of correlative key enzymes in winter wheat under low temperature. Bios Biotechnol Biochem 77:516–525

  • Liu CK, Li YS, Tu BJ, Tian BW, Zhang QY, Liu XB (2016) Effects of potassium fertilizer application on soluble sugar content during reproductive stages and yield in vegetable soybean. Soybean Sci 35:270–274

  • Maathuis FJ (2009) Physiological functions of mineral macronutrients. Curr Opin Plant Biol 12:250–258

  • Mansfield TA, Jones RJ (1971) Effects of abscisic acid on potassium uptake and starch content of stomatal guard cells. Planta 101:147–158

    Article  CAS  PubMed  Google Scholar 

  • Mohamed AI, Rangappa M (1992) Nutrient composition and anti-nutritional factors in vegetable soybean: II. Oil, fatty acids, sterols, and lipoxygenase activity. Food Chem 44:277–282

    Article  CAS  Google Scholar 

  • Nemhauser JL, Hong F, Chory J (2006) Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses. Cell 126:467–475

    Article  CAS  PubMed  Google Scholar 

  • Peuke AD, Jeschke WD, Hartung W (2002) Flows of elements, ions and abscisic acid in Ricinus communis and site of nitrate reduction under potassium limitation. J Exp Bot 53:241–250

    Article  CAS  PubMed  Google Scholar 

  • Qin W, Chen BL, He Q, Si HZ, Li JH (2012) Effects of different ratios of N, P and K fertilizers on endogenous hormone of Xinjiang Fushi apple. J XinJ Agric University 35:373–378 (Chinese with english summary)

    CAS  Google Scholar 

  • Ross JJ, Weston DE, Davidson SE, Reid JB (2011) Plant hormone interactions: how complex are they? Physiol Plant 141:299–309

  • Santner A, Estelle M (2009) Recent advances and emerging trends in plant hormone signalling. Nature 459:1071–1078

    Article  CAS  PubMed  Google Scholar 

  • Stancheva I, Mitova I, Petkova Z (2004) Effects of different nitrogen fertilizer sources on the yield, nitrate content and other physiological parameters in garden beans. Environ Exp Bot 52:277–282

    Article  CAS  Google Scholar 

  • Vreugdenhil D (1983) Abscisic acid inhibits phloem loading of sucrose. Physiol Plant 57:463–467

  • Vyn TJ, Yin X, Bruulsema TW, Jackson CJC, Rajcan I, Brouder SM (2002) Potassium fertilization effects on isoflavone concentrations in soybean [Glycine max (L.) Merr.]. J Agric Food Chem 50:3501–3506

    Article  CAS  PubMed  Google Scholar 

  • Wang YY, Khoo KH, Chen ST, Lin CC, Wong CH, Lin CH (2002) Studies on the immuno-modulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: Functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities. Bioorgan Med Chem 10:1057–1062

    Article  CAS  Google Scholar 

  • Wilkinson S, Davies WJ (2002) ABA-based chemical signalling: the co-ordination of responses to stress in plants. Plant Cell Environ 25:195–210

    Article  CAS  PubMed  Google Scholar 

  • Yang J, Zhang J, Wang Z, Zhu Q, Wang W (2001) Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiol 127:315–323

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yinbo G, Peoples MB, Rerkasem B (1997) The effect of N fertilizer strategy on N 2 fixation, growth and yield of vegetable soybean. Field Crop Res 51:221–229

    Article  Google Scholar 

  • Zeevaart JAD, Creelman RA (1988) Metabolism and physiology of abscisic acid. Annu Rev Plant Physiol Mol Biol 39:439–473

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research is partially funded by National Natural Science Foundation of China (Grant No. 41471241) and Major Program of National Science and Technology of China (2016YFD0102105, 2016YFD0100201).

Author information

Authors and Affiliations

  1. Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Haping Road No. 138, Harbin, 150081, Heilongjiang, China

    Bingjie Tu, Changkai Liu, Bowen Tian, Qiuying Zhang & Xiaobing Liu

  2. College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, China

    Bingjie Tu, Bowen Tian & Xiaobing Liu

  3. Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA, 01003, USA

    Stephen J. Herbert

Authors
  1. Bingjie Tu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Changkai Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bowen Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qiuying Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaobing Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Stephen J. Herbert
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qiuying Zhang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tu, B., Liu, C., Tian, B. et al. Reduced abscisic acid content is responsible for enhanced sucrose accumulation by potassium nutrition in vegetable soybean seeds. J Plant Res 130, 551–558 (2017). https://doi.org/10.1007/s10265-017-0912-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10265-017-0912-x

Keywords

Search

Navigation