Abstract
Candida albicans is a commensal yeast in humans that disseminates in immunocompromised persons. Its spreading is modulated by melanin, hormones, or some neurotransmitters, among other factors. The neurotransmitter gamma-aminobutyric acid (GABA) is used by bacteria, plants, and fungi as a carbon and nitrogen source. In this article, the in vitro effect of different doses of GABA on germ-tube formation and expression of phospholipase B1 (PLB1) mRNA in two Candida albicans strains was investigated. Results demonstrated that GABA increases both germ-tube formation and PLB1 mRNA expression in the two Candida strains in a dose-dependent manner, which suggests that GABA promotes the growth of C. albicans.
References
Akpan A, Morgan R (2002) Oral candidiasis. Postgrad Med J 78:455–459
Buvanendran A, Kroin JS, Kari M, Tuman KJ (2010) Can a single dose of 300 mg of pregabalin reach acute antihyperalgesic levels in the central nervous system? Reg Anesth Pain Med 35:535–538
Calderone RA, Fonzi WA (2001) Virulence factors of Candida albicans. Trends Microbiol 9:327–335
Chevrot R, Rosen R, Haudecoeur E, Corou A, Shelp BJ, Ron E, Faure D (2006) GABA controls the level of quorum-sensing signal in Agrobacterium tumefaciens. Proc Natl Acad Sci USA 103:7460–7464
Collart MA, Oliviero S (2001) Preparation of yeast RNA. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. Wiley, New York, pp 13.12.1–13.12.5
d’Ostiani C, del Sero G, Bacci A, Montagnoli C, Spreca A, Mencacci A, Ricciardi-Castagnoli P, Romani L (2000) Dendritic cell discrimination between yeast and hyphae of the fungus Candida albicans: implications for initiation of T helper cell immunity in vitro and in vivo. J Exp Med 191:1661–1674
Dotis J, Roilides E (2007) Immunopathogenesis of central nervous system fungal infections. Neurol India 55:216–220
Gabler IG, Barbosa AC, Velela RR, Lyon S, Rosa CA (2008) Incidence and anatomical localization of oral candidiasis in patients with AIDS hospitalized in a public hospital in Belo Horizonte, MG, Brazil. J Appl Oral Sci 16:247–250
Kinnersley AM, Lin F (2000) Receptor modifiers indicate that 4-aminobutyric acid (GABA) is a potential modulator of ion transport in plants. Plant Growth Regul 32:65–76
Larsen B, Anderson S, Brockman A, Essmann M, Schmidt M (2006) Key physiological differences in Candida albicans CDR1 induction by steroid hormones and antifungal drugs. Yeast 23:795–802
Lubick K, Miranda R, Jutila M (2007) Securinine, a GABAA receptor antagonist, enhances macrophage clearance of phase II C. burnetii: comparison with TLR agonists. J Leukoc Biol 82:1062–1069
Luo G, Mitchell TG (2002) Rapid identification of pathogenic fungi directly from cultures by using multiplex PCR. J Clin Microbiol 40:2860–2865
Macreadie IG, Bartone N, Sparrow L (2010) Inhibition of respiratory growth and survival in yeast by dopamine and counteraction with ascorbate or glutathione. J Biomol Screen 15:297–301
Mayr A, Hinterberger G, Dierich MP, Lass-Flörl C (2005) Interaction of serotonin with Candida albicans selectively attenuates fungal virulence in vitro. Int J Antimicrob Agents 26:335–337
McNemar MD, Gorman JA, Buckley HR (2001) Isolation of a gene coding a putative polyamine transporter from Candida albicans, GPT1. Yeast 18:555–561
Morris-Jones R, Gomez BL, Diez S, Uran M, Morris-Jones SD, Casadevall A, Nosanchuk JD, Hamilton AJ (2005) Synthesis of melanin pigment by Candida albicans in vitro and during infection. Infect Immun 73:6147–6150
Mukherjee PK, Chandra J, Kuhn DM, Ghannoum MA (2003) Differential expression of Candida albicans phospholipase B (PLB1) under various environmental and physiological conditions. Microbiology 149:261–267
Okeke CN, Tsuboi R, Kawai M, Hiruma M, Ogawa H (2001) Isolation of an intron-containing partial sequence of the gene encoding dermatophyte actin (ACT) and detection of a fragment of the transcript by reverse transcription-nested PCR as a means of assessing the viability of dermatophytes in skin scales. J Clin Microbiol 39:101–106
Owens DF, Kriegstein AR (2002) Is there more to GABA than synaptic inhibition? Nat Rev Neurosci 3:715–727
Ramos F, el Guezzar M, Grenson M, Wiame JM (1985) Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae. Eur J Biochem 149:401–404
Reiss D, Quesneville H, Nouaud D, Andrieu O, Anxolabehere D (2003) Hoppel, a P-like element without introns: a P-element ancestral structure or a retrotranscription derivative? Mol Biol Evol 20:869–879
Reyes-García MG, Hernández-Hernández F, Hernández-Tellez B, García-Tamayo F (2007) GABA (A) receptor subunits RNA expression in mice peritoneal macrophages modulates their IL-6/IL-12 production. J Neuroimmunol 188:64–68
Rodaki A, Bohovych IM, Enjalbert B, Young T, Odds FC, Gow NA, Brown AJ (2009) Glucose promotes stress resistance in the fungal pathogen Candida albicans. Mol Biol Cell 20:4845–4855
Selmecki A, Forche A, Berman J (2010) Genomic plasticity of the human fungal pathogen Candida albicans. Eukaryot Cell 9:991–1008
Solomon PS, Oliver RP (2002) Evidence that gamma-aminobutyric acid is a major nitrogen source during Cladosporium fulvum infection of tomato. Planta (Berl) 214:414–420
Zhang H, Tang M, Chen H (2010) Growth promotion of Suillus luteus by adenosine in vitro. Mycoscience 51:139–143
Acknowledgments
We thank Joseph Doshner-Maute for correcting the English version of the manuscript. The authors have no conflict of interest. We declare that all experiments in this paper comply with the current laws of Mexico.
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Reyes-García, M.G., Hernández-Hernández, F. & García-Tamayo, F. Gamma-aminobutyric acid (GABA) increases in vitro germ-tube formation and phospholipase B1 mRNA expression in Candida albicans . Mycoscience 53, 36–39 (2012). https://doi.org/10.1007/s10267-011-0130-7
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DOI: https://doi.org/10.1007/s10267-011-0130-7