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LC Method for the Determination of Rhaponticin in Rat Plasma, Faeces and Urine for Application to Pharmacokinetic Studies

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Abstract

A simple liquid chromatography (LC) method has been developed and validated to determine rhaponticin in rat plasma, faeces and urine. Chromatographic separation was achieved through mobile phase consisting of acetonitrile and water at a flow rate of 1.0 mL min−1. Rhaponticin was quantified using UV detection at 324 nm. The assay was linear over the concentration range of 50–4,000 ng mL−1 for plasma, faeces and urine. The intra- and inter-day RSD were less than 10%. The plasma, faeces and urine rhaponticin levels were monitored in rats after oral administration. This simple LC method appears to be useful in the pharmacokinetic investigation of rhaponticin.

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Acknowledgements

This study was supported in part by grants from the National Scientific Foundation of China (No. 81001622).

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Authors and Affiliations

  1. Biomedicine Key Laboratory of Shaanxi Province, The College of Life Sciences, Northwest University, No.229 Taibai North Road, Xi’an, 710069, Shaanxi, China

    Yingyong Zhao, Lili Wang, Jing Li, Ruichao Lin & Wenji Sun

  2. Department of Nutrition, University of California, One Shields Avenue, Davis, CA, 95616-8669, USA

    Charlene C. Ho

  3. National Institute for the Control of Pharmaceutical and Biological Products, Beijing, 100050, China

    Xianlong Cheng & Ruichao Lin

  4. Nanyang Normal University, No.1 Wolong Road, Nanyang, 473000, Henan, China

    Hua Miao

  5. Institut Parisien de Chimie Moléculaire, UMR CNRS 7201, Université Pierre et Marie Curie-Paris 6, 4 place Jussieu, 75005, Paris, France

    Yongmin Zhang

Authors
  1. Yingyong Zhao
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  2. Lili Wang
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  3. Hua Miao
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  4. Charlene C. Ho
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  5. Jing Li
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  6. Xianlong Cheng
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  7. Yongmin Zhang
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  8. Ruichao Lin
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  9. Wenji Sun
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Corresponding author

Correspondence to Wenji Sun.

Additional information

Y.-Y. Zhao and L.-L. Wang are co-first authors.

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Zhao, Y., Wang, L., Miao, H. et al. LC Method for the Determination of Rhaponticin in Rat Plasma, Faeces and Urine for Application to Pharmacokinetic Studies. Chromatographia 73, 599–603 (2011). https://doi.org/10.1007/s10337-011-1914-9

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  • DOI: https://doi.org/10.1007/s10337-011-1914-9

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