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Agromyces indicus sp. nov., isolated from mangroves sediment in Chorao Island, Goa, India

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Abstract

A Gram-positive, non-motile, rod-shaped bacterium strain NIO-1018T isolated from a mangrove sediment sample of the Chorao Island, Goa, India, was subjected to a detailed polyphasic taxonomic study. The strain designated as NIO-1018T matched with most of the phenotypic and chemotaxonomic properties of the genus Agromyces and represents a novel species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1018T fell within the cluster comprising species of the genus Agromyces, clustering with Agromyces soli (98.1 %), Agromyces flavus (97.9 %), Agromyces aurantiacus (97.7 %) and Agromyces ulmi (97.3 %). The predominant menaquinone was MK-12, and the major cellular fatty acids were anteiso-C15:0, iso-C16:0, anteiso-C17:0 and iso-C15:0. The polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol. The genomic DNA G+C content of strain NIO-1018T was 71.8 mol%. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain NIO-1018T represents a novel species of the genus Agromyces, for which the name Agromyces indicus sp. nov. is proposed. The type strain is NIO-1018 (=JCM 17573T = CCTCC AB2011122T).

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Acknowledgments

Author thanks Dr. S. R. Shetye, Director, National Institute of Oceanography for facilities and encouragement. This work was supported from the grants under OLP0001 from the Council for Scientific and Industrial Research (CSIR), New Delhi. This is NIO contribution number 5162.

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Authors and Affiliations

  1. Biological Oceanography Division, National Institute of Oceanography (CSIR), National Institute of Oceanography (CSIR Lab), Dona Paula, 403004, Goa, India

    Syed G. Dastager & Samir Damare

  2. Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Zheng-Liu Qiang, Shu-Kun Tang & Wen-Jun Li

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  1. Syed G. Dastager
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  2. Zheng-Liu Qiang
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  3. Samir Damare
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Correspondence to Syed G. Dastager.

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Dastager, S.G., Qiang, ZL., Damare, S. et al. Agromyces indicus sp. nov., isolated from mangroves sediment in Chorao Island, Goa, India. Antonie van Leeuwenhoek 102, 345–352 (2012). https://doi.org/10.1007/s10482-012-9744-9

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