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Streptomyces alkalithermotolerans sp. nov., a novel alkaliphilic and thermotolerant actinomycete isolated from a soda lake

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Abstract

An alkaliphilic actinomycete, strain AC3T, was isolated from Lonar soda lake, in India. Based on 16S rRNA gene sequence analysis it was identified that the strain belongs to the class Actinobacteria and was most closely related to Streptomyces sodiiphilus JCM 13581T (96.4 % sequence similarity), Streptomyces leeuwenhoekii DSM 42122T (96.1 %), Streptomyces albus NRRL B-2365T (96.1 %), Streptomyces panacagri Gsoil 519T (96.0 %), Streptomyces fimbriatus NBRC 15411T (95.9 %) and other members of the genus Streptomyces (<95.9 %).The strain formed a well separated clade in the genus Streptomyces based on the 16S rRNA gene analysis of all of the validly named Streptomyces species. Strain AC3T was observed to produce light-cream substrate and white aerial mycelia on most tested media. The optimum pH for growth was determined to be 9.5–10.0 with no growth at pH 7.0. The DNA G+C content of strain AC3T was determined to be 71.2 mol %. The results of the polyphasic analysis allowed a clear differentiation of strain AC3T from all other members of the genus Streptomyces. Strain AC3T is thus considered to represent a novel member of the genus Streptomyces, for which the name Streptomyces alkalithermotolerans sp. nov. is proposed. The type strain is AC3T (=KCTC 29497T = JCM 30167T).

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References

  • Antony-Babu S, Goodfellow M (2008) Biosystematics of alkaliphilic streptomycetes isolated from seven locations across a beach and dune sand system. Antonie Van Leeuwenhoek 94:581–591

    Article  PubMed  Google Scholar 

  • Bahn YS, Park JM, Bai DH, TakaseS YuJH (1998) YUA001, a novel aldose reductase inhibitor isolated from alkalophilic Corynebacterium sp. YUA25. I. Taxonomy, fermentation, isolation and characterization. J Antibiot 51:902–907

    Article  CAS  PubMed  Google Scholar 

  • Bérdy J (2005) Bioactive microbial metabolites: a personal view. J Antibiot 58:1–26

    Article  PubMed  Google Scholar 

  • Bérdy J (2012) Thoughts and facts about antibiotics: where we are now and where we are heading. J Antibiot 65:385–395

    Article  PubMed  Google Scholar 

  • Collins MD (1985) Isoprenoid quinone analysis in classification and identification. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 267–287

    Google Scholar 

  • Duckworth AW, Grant S, Grant WD, Jones BE, Meijer D (1998) Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an East African soda lake. Extremophiles 2:359–366

    Article  CAS  PubMed  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Goodfellow M, Fiedler HP (2010) A guide to successful bioprospecting: informed by actinobacterial systematics. AntonieVan Leeuwenhoek 98:119–142

    Article  Google Scholar 

  • Groth I, Schumann P, Rainey FA, Martin K, Schuetze B, Augsten K (1997) Bogoriella caseilytica gen. nov., sp. nov., a newalkaliphilic actinomycete from a soda lake in Africa. Int J SystBacteriol 47:788–794

    Article  CAS  Google Scholar 

  • He L, Li W, Huang Y, Wang L, Liu Z, Lanoot B et al (2005) Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Bacteriol 55:1939–1944

    Article  CAS  Google Scholar 

  • Helaly SE, Goodfellow M, Zinecker H, Imhoff JF, Süssmuth RD, Fiedler H-P (2013) Warkmycin, a novel angucycline antibiotic produced by Streptomyces sp. Acta 2930. J Antibiot 66:669–674

    Article  CAS  PubMed  Google Scholar 

  • Horikoshi K (1999) Alkaliphiles: some applications of their products for biotechnology. Microbiol Mol Biol Rev 63:735–750

    CAS  PubMed Central  PubMed  Google Scholar 

  • Huang Y, Li W, Wang L, Lanoot B, Vancanneyt M, Rodriguez C et al (2004) Streptomyces glauciniger sp. nov., a novelmesophilic streptomycete isolated from soil in southChina. Int J Syst Evol Microbiol 54:2085–2089

    Article  CAS  PubMed  Google Scholar 

  • Jones BE, Grant WD, Duckworth AW, Owenson GG (1998) Microbial diversity of soda lakes. Extremophiles 2:191–200

    Article  CAS  PubMed  Google Scholar 

  • Kelly KL (1964) Inter-Society Color Council-National Bureau of Standard Color-name charts illustrated with centroid colors. US Government Printing Office, Washington

    Google Scholar 

  • Kim B, Sahin N, Minnikin DE, Zakrzewska-Czerwinska J, Mordarski M, Goodfellow M (1999) Classification of thermophilic streptomycetes, including the description of Streptomyces thermoalcalitolerans sp. nov. Int J SystEvol Microbiol 49:7–17

    Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  CAS  PubMed  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Kin SL (2006) Discovery of novel metabolites from marine actinomycetes. Curr Opin Microbiol 9:245–251

    Article  Google Scholar 

  • Kroppenstedt RM (1985) Fatty acid and menaquinones analysis of actinomycetes and related organisms. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 173–199

    Google Scholar 

  • Kűster E, Williams ST (1964) Selection of media for isolation of streptomycetes. Nature 202:928–929

    Article  Google Scholar 

  • Lechevalier HA, Lechevalier MP (1970a) A critical evaluation of the genera of aerobic actinomycetes. In: Prauser H (ed) The actinomycetales. VEB Gustav Fischer, Jena, pp 393–405

    Google Scholar 

  • Lechevalier MP, Lechevalier HA (1970b) Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443

    Article  CAS  Google Scholar 

  • Lechevalier MP, Lechevalier HA (1980) The chemotaxonomy of actinomycetes. In: Dietz A, Thayer DW (eds) Actinomycete taxonomy. Society for Industrial Microbiology, Arlington, p 22

    Google Scholar 

  • Li WJ, Zhang YG, Zhang YQ, Tang SK, Xu P, Xu LH, Jiang CL (2005) Streptomyces sodiiphilus sp.nov., a novel alkaliphilic actinomycete. Int J Syst Evol Microbiol 55(3):1329–1333

    Article  CAS  PubMed  Google Scholar 

  • Mao J, Tang Q, Zhang Z, Wang W, Wei D, Huang Y et al (2007) Streptomyces radiopugnans sp. nov., a radiation resistant actinomycete isolated from radiation-polluted soil in China. Int J Syst Evol Microbiol 57:2578–2582

    Article  CAS  PubMed  Google Scholar 

  • Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218

    Article  CAS  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  • Mikami Y, Miyashita K, Arai T (1982) Diaminopimelic acidprofiles of alkalophilic and alkaline-resistant strains of actinomycetes. J Gen Microbiol 128:1709–1712

    CAS  PubMed  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal K, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, New York

    Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Inc, Newark

    Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  • Shirling EB, Gottlieb D (1968a) Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study. Int J Syst Bacteriol 18:69–189

    Article  Google Scholar 

  • Shirling EB, Gottlieb D (1968b) Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. Int J Syst Bacteriol 18:279–392

    Article  Google Scholar 

  • Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231

    CAS  PubMed Central  PubMed  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis (MEGA) using maximum likelihood, evoluttionary distance and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Tsujibo H, Sakamoto T, Kusano G, Miyamoto K, Ogura M, Hasegawa T, Inamori Y (1990) Isolation of cytotoxic substance, kalafungin from an alkalophilic actinomycete, Nocardiopsis dassonvillei subsp. prasina. Chem Pharm Bull (Tokyo) 38:2299–2300

    Article  CAS  Google Scholar 

  • Vishnuvardhan Reddy S, Aspana S, Tushar DL, Sasikala CH, Ramana V (2013) Spirochaeta sphaeroplastigenens sp. nov., a halo-alkaliphilic, obligately anaerobic spirochaete isolated from soda lake Lonar. Int J Syst Evol Microbiol 63:2223–2228

    Article  PubMed  Google Scholar 

  • Williams ST, Goodfellow M, Alderson G, Wellington EMH, Sneath PHA, Sackin MJ (1983) Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813

    CAS  PubMed  Google Scholar 

  • Zhang Y-G, Wang H-F, Liu Q, Hozzein WN, Wadaan MA, Cheng J, Yue-Ji Chen, Yuan-Ming Zhang, Li WJ (2013) Streptomyces fukangensis sp. nov., a novel alkaliphilic actinomycete isolated from a saline-alkaline soil. Antonie Van Leeuwenhoek 104:1227–1233

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank Dr. A.C. Parte for the etymology and Latin description of the species epithet. SVR and MT thank Department of Science & Technology, Government of India, for providing FTSYS project grants (SB/FT/LS-115/2012-13 and SB/FT/LS-320/2012-13, respectively). FM thanks Department of Science & Technology, Government of India, for providing PA fellowship.

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Authors and Affiliations

  1. Microbial Ecology Lab, Department of Biochemistry, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda, 508254, India

    Vishnuvardhan Reddy Sultanpuram, Thirumala Mothe & Farooq Mohammed

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  1. Vishnuvardhan Reddy Sultanpuram
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  2. Thirumala Mothe
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  3. Farooq Mohammed
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Correspondence to Vishnuvardhan Reddy Sultanpuram.

Electronic supplementary material

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10482_2014_332_MOESM1_ESM.docx

Supplementary material 1 (DOCX 67 kb) Supplementary Fig. 1 Whole cell amino acids extract TLC of strain AC3T and Streptomyces sodiiphilus JCM 13581T. This was developed in methanol:pyridine:water:12 N HCl (32:4:7:1 v/v)

10482_2014_332_MOESM2_ESM.docx

Supplementary material 2 (DOCX 74 kb) Supplementary Fig. 2 Whole cell sugars extract TLC of strain AC3T and Streptomyces sodiiphilus JCM 13581T. This was developed in butanol:pyridine:water (50:30:20 v/v)

10482_2014_332_MOESM3_ESM.docx

Supplementary material 3 (DOCX 363 kb) Supplementary Fig. 3. Two-dimensional thin-layer chromatogram of polar lipid extracts of AC3T and Streptomyces sodiiphilus JCM 13581T. The first direction was developed in CHCl3:CH3OH:H2O (75:32:4 by volume) and the second in CHCl3:CH3OH:CH3COOH:H2O (86:16:15:4 by volume). PG, phosphatidylglycerol; GL-glycolipid; PE, phosphatidylethanolamine; PL1-3, phospholipids

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Sultanpuram, V.R., Mothe, T. & Mohammed, F. Streptomyces alkalithermotolerans sp. nov., a novel alkaliphilic and thermotolerant actinomycete isolated from a soda lake. Antonie van Leeuwenhoek 107, 337–344 (2015). https://doi.org/10.1007/s10482-014-0332-z

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  • DOI: https://doi.org/10.1007/s10482-014-0332-z

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