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Okibacterium endophyticum sp. nov., a novel endophytic actinobacterium isolated from roots of Salsola affinis C. A. Mey

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Abstract

A white bacterial strain, designated EGI 650022T, was isolated from the roots of Salsola affinis C. A. Mey, collected from Urumqi City, Xinjiang, north-western China. The strain was found to be aerobic, Gram-stain positive, oxidase-positive and catalase-positive. Cells were non-motile and irregular rods. Growth occurred at NaCl concentrations between 0 and 7 % (w/v), temperatures between 5 and 45 °C, and pH 6.0–9.0. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain EGI 650022T belongs to a clade with the genera Okibacterium and Plantibacter in the family Microbacteriaceae. The novel strain EGI 650022T showed highest levels of 16S rRNA gene sequence similarity with members of the genera Okibacterium and Plantibacter (97.2–98.0 %). The cell-wall peptidoglycan contained glutamate, homoserine, glycine, alanine and lysine. The predominant menaquinones (MKs) were MK-11, MK-12 and MK-12 (H4). The polar lipid pattern comprised phosphatidylglycerol, diphosphatidylglycerol, two unknown glycolipids and two unknown phospholipids. The major fatty acids were anteiso-C15:0 and anteiso-C17:0. The DNA G+C content was 66.0 mol%. The DNA–DNA relatedness values of strain EGI 650022T with Okibacterium fritillariae DSM 12584T, Plantibacter flavus DSM 14012T and Plantibacter auratus DSM 19586T were 39.7, 19.7 and 22.0 %. Based on phylogenetic, phenotypic, chemotaxonomic and DNA–DNA hybridization data, strain EGI 650022T is considered to represent a novel species of the genus Okibacterium, for which the name Okibacterium endophyticum sp. nov. is proposed; the type strain is EGI 650022T (=JCM 30086T = KCTC 29492T).

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Acknowledgments

The authors are grateful to Prof. Dr. Aharon Oren (The Hebrew University of Jerusalem, Israel) for his kind help with the Latin etymology for the new species, Prof. Hans-Peter Klenk (DSMZ, Germany) and Prof. Takuji Kudo (JCM, Japan) for their kindly providing reference type strains. This research was supported by the National Natural Science Foundation of China (Nos. 81102806, 31200008 and 31400009). The Hundred Talents Program of Chinese Academy of Sciences, the High-level Talents Program of Xinjiang Autonomous Region and the West Light Foundation of Chinese Academy of Sciences. WNH and W-J L extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no. RGP-205. W-J Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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Authors and Affiliations

  1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, People’s Republic of China

    Hong-Fei Wang, Yong-Guang Zhang, Li Li, Ji-Yue Chen, Jian-Wei Guo, Yuan-Ming Zhang & Wen-Jun Li

  2. State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Yong-Guang Zhang & Wen-Jun Li

  3. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Wen-Jun Li

  4. Department of Agriculture and Biological Sciences, Dali University, Dali, 671003, People’s Republic of China

    Wei-Hong Liu

  5. Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Wael N. Hozzein

  6. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Hong-Fei Wang

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  1. Hong-Fei Wang
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Correspondence to Wen-Jun Li.

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Wang, HF., Zhang, YG., Li, L. et al. Okibacterium endophyticum sp. nov., a novel endophytic actinobacterium isolated from roots of Salsola affinis C. A. Mey. Antonie van Leeuwenhoek 107, 835–843 (2015). https://doi.org/10.1007/s10482-014-0376-0

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