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Pseudovibrio hongkongensis sp. nov., isolated from a marine flatworm

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Abstract

A Gram-negative, motile, rod-shaped bacterial strain, designated UST20140214-015BT, was isolated from a marine flatworm (Polyclad). The bacterium was found to be Gram-negative, oxidase and catalase positive, long-rod shaped, and motile by means of several peritrichous or subpolar flagella. Strain UST20140214-015BT grew optimally at 28 °C, at pH 7.0 and in the presence of 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain UST20140214-015BT belongs to the genus Pseudovibrio, with highest sequence similarity to Pseudovibrio japonicus WSF2T (96.9 %), followed by P. ascidiaceicola F423T (96.7 %), P. denitrificans DN34T (96.6 %), P. axinellae Ad2T (96.3 %). All the other species shared <95.5 % sequence similarity. The principal fatty acids were identified as summed feature 8 (C18:1 ω7c/ω6c, as defined by the MIDI system; 70.8 %), C16:0 (9.1 %), summed feature 3 (C16:1 ω6c/C16:1 ω7c; 5.7 %). The G+C content of the chromosomal DNA was determined to be 53.3 mol%. The combined genotypic and phenotypic data show that strain UST20140214-015BT represents a novel species within the genus Pseudovibrio, for which the name Pseudovibrio hongkongensis sp. nov. is proposed, with the type strain UST20140214-015BT (=KCTC 42383T = MCCC 1K00451T).

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Abbreviations

MCCC:

Marine Culture Collection of China

KCTC:

Korean Collection for Type Cultures

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Acknowledgments

This work was financially supported by the National High Technology Research and Development Program of China (863 program, No. 2012AA092103) and Seed Funding from Scientific and Technical Innovation Council of Shenzhen Government (Grant No. 827000012).

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    Authors and Affiliations

    1. Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen, 518060, China

      Ying Xu & Yu Zhang

    2. Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China

      Ying Xu, Qi Li & Renmao Tian

    3. Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration, Xiamen, 361005, China

      Qiliang Lai

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    1. Ying Xu
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    2. Qi Li
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    Correspondence to Yu Zhang.

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    Ying Xu, Qi Li are contributed equally to this work.

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    Xu, Y., Li, Q., Tian, R. et al. Pseudovibrio hongkongensis sp. nov., isolated from a marine flatworm. Antonie van Leeuwenhoek 108, 127–132 (2015). https://doi.org/10.1007/s10482-015-0470-y

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