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Lampropedia puyangensis sp. nov., isolated from symptomatic bark of Populus × euramericana canker and emended description of Lampropedia hyalina (Ehrenberg 1832) Lee et al. 2004

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Abstract

A Gram-stain negative, Neisser-stain negative, aerobic, non-motile, non-spore-forming, slimy, glossy bacterial strain with single or clustered coccoid cells and white colony colour, designated as 2-binT, was isolated from cankered bark tissue of Populus × euramericana. The strain was found to grow at 15–40 °C and pH 5–10, with an optimum of 30 °C and pH 8.0. The strain was found to be negative with respect to catalase and positive for oxidase activity, nitrate reduction and Voges–Proskauer reaction. Analysis of 16S rRNA gene sequence data indicated that the isolate belongs to the genus Lampropedia, having sequence similarity of 96.24 % with Lampropedia hyalina ATCC11041T. DNA–DNA relatedness of strain 2-binT with L. hyalina JCM 21380T was 26.7 ± 4.6 %. The DNA G+C content of strain 2-binT was determined to be 57 % and the major cellular fatty acids were identified as C16:0, C16:1 ω7c/C16:1 ω6c and C18:1 ω7c. The polar lipid profile of strain 2-binT was found to contain diphosphatidylglycerol, a glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, a phospholipid, phosphatidylmonomethylethanolamine and three unidentified lipids (L1, L2, L3). Based on molecular data and physiological and biochemical characteristics, strain 2-binT is considered to represent a novel species in the genus Lampropedia, for which the name Lampropedia puyangensis sp. nov. is proposed. The type strain is 2-binT (= CFCC 10925T = KCTC 32235T).

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References

  • Austin JW, Murray RG (1990) Isolation and in vitro assembly of the components of the outer S layer of Lampropedia hyalina. J Bacteriol 172:3681–3689

    CAS  PubMed Central  PubMed  Google Scholar 

  • De Ley J, Cattoir H, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142

    Article  PubMed  Google Scholar 

  • Del Sal G, Manfioletti G, Schneider C (1989) The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages or plasmids suitable for sequencing. Biotechniques 7:514–520

    PubMed  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Forbes L (1981) Rapid flagella stain. J Clin Microbiol 13:807–809

    CAS  PubMed Central  PubMed  Google Scholar 

  • Jenkins D, Richard MG, Daigger GT (1986) Manual on the causes and control of activated sludge bulking and foaming. Water Research Commission

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH et al (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  CAS  PubMed  Google Scholar 

  • Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Chichester, Wiley, pp 115–175

    Google Scholar 

  • Lee N, Cellamare CM, Bastianutti C, Rosselló-Mora R, Kämpfer P, Ludwig W, Schleifer K, Stante L (2004) Emended description of the species Lampropedia hyalina. Int J Syst Evol Microbiol 54:1709–1715

    Article  CAS  PubMed  Google Scholar 

  • Li Y, Piao CG, Ma YC, He W et al (2013) Acinetobacter puyangensis sp. nov., isolated from the healthy and diseased part of Populus × euramericana canker bark. Int J Syst Evol Microbiol 63:2963–2969

    Article  CAS  PubMed  Google Scholar 

  • Li Y, He W, Ren FJ, Guo LM et al (2014a) A canker disease of Populus × euramericana in China, caused by Lonsdalea quercina subsp. populi. Plant Dis 98:368–378

    Article  CAS  Google Scholar 

  • Li Y, He W, Wang T, Piao CG et al (2014b) Acinetobacter qingfengensis sp. nov., isolated from Populus × euramericana canker bark. Int J Syst Evol Microbiol 64:3417–3421

    Article  Google Scholar 

  • Li Y, Fang W, Xue H, Liang WX, Wang LF, Tian Z.G et al (2014c) Brenneria populi sp. nov., isolated from symptomatic bark of Populus × euramericana canker. Int J Syst Evol Microbiol Nov 10. doi:10.1099/ijs.0.066068-0

  • Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218

    Article  CAS  Google Scholar 

  • Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118

    Article  CAS  PubMed  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M et al (1984) An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Metheods 2:233–241

    Article  CAS  Google Scholar 

  • Murray RGE (1984) Genus Lampropedia Schoreter 1886, 151AL. In: NR Krieg, JG Holt (eds) Bergey’s Manual of Systematic Bacteriology. Baltimore: Williams & Wilkins, vol. 1, pp 402–406

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Inc, Newark

    Google Scholar 

  • Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Manual of methods for general and bacteriology. American Society for Microbiology, Washington DC, pp 607–654

    Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH et al (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Willems A, De Ley J, Gillis M, Kersters K (1991) Comamonadaceae, a new family encompassing the acidovorans rRNA complex, including Variovorax paradoxus gen. nov., comb. nov., for Alcaligenes paradoxus (Davis) 1969. Int J Syst Bacteriol 41:445–450

    Article  Google Scholar 

  • Xie CH, Yokota A (2003) Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA sequence. J Gen Appl Microbiol 49:345–349

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The research was supported by the Special Fund of the Chinese Central Government for Basic Scientific Research Operations in Commonweal Research Institutes (CAFRIFEEP201102-4) and National Infrastructure of Microbial Resources (NIMR-2013-7). We also thank Professor Everett M. Hansen for his help with the written English language.

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    Authors and Affiliations

    1. The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing, 100091, China

      Yong Li, Tao Wang, Chun-gen Piao, Lai-fa Wang, Guo-zhong Tian & Min-wei Guo

    2. College of Forestry, Sichuan Agricultural University, Ya’an, 625014, China

      Tao Wang & Tian-hui Zhu

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    1. Yong Li
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    2. Tao Wang
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    3. Chun-gen Piao
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    Correspondence to Chun-gen Piao.

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    Yong Li and Tao Wang have contributed equally to this work.

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    Li, Y., Wang, T., Piao, Cg. et al. Lampropedia puyangensis sp. nov., isolated from symptomatic bark of Populus × euramericana canker and emended description of Lampropedia hyalina (Ehrenberg 1832) Lee et al. 2004. Antonie van Leeuwenhoek 108, 321–328 (2015). https://doi.org/10.1007/s10482-015-0483-6

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