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Vibrio mexicanus sp. nov., isolated from a cultured oyster Crassostrea corteziensis

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Abstract

A bacterial strain was taxonomically characterised by means of a genomic approach comprising 16S rRNA gene sequence analysis, multilocus sequence analysis (MLSA), the DNA G+C content, whole genome analyses (ANI and GGDC) and phenotypic characterisation. The strain CAIM 1540T was isolated from a cultured oyster Crassostrea corteziensis in La Cruz, Sinaloa state, México. The isolate was found to be catalase and oxidase positive, cells were observed to be motile, O/129-sensitive and facultatively anaerobic. The almost-complete 16S rRNA gene sequence placed this strain within the genus Vibrio; the closest related species were found to be Vibrio aestivus, Vibrio marisflavi, Vibrio maritimus and Vibrio variabilis with similarity values of 99.02, 97.05, 96.70, and 96.59 % respectively. MLSA of four housekeeping genes (ftsZ, gapA, recA, and topA) was performed with the closely related species. A draft genome sequence of strain CAIM 1540T was obtained. The DNA G+C content of this strain was determined to be 43.7 mol%.The ANI values with V. aestivus were 89.6 % (ANIb), 90.6 % (ANIm) and a GGDC value of 39.5 ± 2.5 % was obtained; with V. marisflavi the genomic similarities were 71.5 % (ANIb), 85.5 % (ANIm) and 20.2 ± 2.3 % (GGDC); with V. maritimus 72.6 % (ANIb), 85.7 % (ANIm) and 22.0 ± 2.0 % (GGDC); and with V. variabilis 72.6 % (ANIb), 85.8 % (ANIm) and 21.6 ± 1.6 % (GGDC). These ANI and GGDC values are below the threshold for the delimitation of prokaryotic species, i.e. 95–96 and 70 %, respectively. Phenotypic characters also showed differences with the closest related species analysed. The results presented here support the description of a novel species, for which the name Vibrio mexicanus sp. nov. is proposed, with strain CAIM 1540T (= CECT 8828T, = DSM 100338T) as the type strain. In addition, we found that the recently described species Vibrio thalassae and Vibrio madracius might be a single species because the values of ANIb 95.8 %, ANIm 96.6 % and GGDC 70.2 ± 2.9 % are above the accepted species thresholds.

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Abbreviations

ANI:

Average nucleotide identity

GGDC:

Genome-to-genome distance calculator

MLSA:

Multilocus sequence analysis

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Acknowledgments

This work was partially funded by CONACYT project CB-2009-01 132328 and Grant AGL–2010–18438 from the Ministerio de Ciencia e Innovación (Spain).

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Authors and Affiliations

  1. CIAD, A.C., Mazatlán Unit for Aquaculture, AP. 711, 82000, Mazatlán, Sinaloa, Mexico

    Adrián González-Castillo, Julissa Enciso-Ibarrra, M. Carmen Bolán-Mejia & Bruno Gomez-Gil

  2. Departamento de Microbiología y Parasitología, CIBUS, Universidad de Santiago de Compostela, Campus Sur s/n, 15782, Santiago de Compostela, Spain

    Sabela Balboa, Aide Lasa & Jesús L. Romalde

  3. Instituto Tecnológico de Tepic, Av. Tecnológico 2595, 63175, Tepic, Nayarit, Mexico

    Hector Cabanillas-Beltrán

Authors
  1. Adrián González-Castillo
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  2. Julissa Enciso-Ibarrra
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  3. M. Carmen Bolán-Mejia
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  4. Sabela Balboa
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  5. Aide Lasa
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  6. Jesús L. Romalde
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  7. Hector Cabanillas-Beltrán
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  8. Bruno Gomez-Gil
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Correspondence to Bruno Gomez-Gil.

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González-Castillo, A., Enciso-Ibarrra, J., Bolán-Mejia, M.C. et al. Vibrio mexicanus sp. nov., isolated from a cultured oyster Crassostrea corteziensis . Antonie van Leeuwenhoek 108, 355–364 (2015). https://doi.org/10.1007/s10482-015-0488-1

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