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Aquamicrobium soli sp. nov., a bacterium isolated from a chlorobenzoate-contaminated soil

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Abstract

An aerobic, Gram-stain negative, short rod-shaped, asporogenous, non-motile bacterium designated strain NK8T was isolated from a chlorobenzoate contaminated soil in China. Strain NK8T was observed to grow optimally at pH 7.0, 30 °C and in the absence of NaCl in LB medium. The G + C content of the total DNA of strain NK8T was found to be 65.5 mol%. The 16S rRNA gene sequence of strain NK8T showed high similarity to that of Aquamicrobium aerolatum Sa14T (97.3%), followed by Aquamicrobium lusatiense S1T (96.7%) and Mesorhizobium sangali SCAU7T (96.6%). The DNA–DNA relatedness between strain NK8T and A. aerolatum Sa14T was 35.5 ± 0.9%. The major fatty acids of strain NK8T were determined to be C19:0 cyclo ω8c (45.6%), C18:1 ω7c (33.4%) and C16:0 (8.4%). The respiratory quinone was found to be ubiquinone Q-10. The major polyamine was found to be spermidine. The polar lipid profile include the major compounds phosphatidylcholine and diphosphatidylglycerol, and moderate amounts of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, aminolipid and phospholipid. Based on the differential biochemical and physiological characteristics, the geno-, chemo- and phenotypic characteristics, strain NK8T is proposed to represent a novel species of the genus Aquamicrobium, Aquamicrobium soli sp. nov. The type strain is NK8T (=KCTC 52165T=CCTCC AB2016045T).

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Acknowledgements

This work was supported by the Fundamental Research Funds for the Central Universities (KYZ201422), the Outstanding Youth Foundation of Jiangsu Province (BK20130029) and the Scientific Foundation from Yunnan Tobacco Company (2014YN08).

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Authors and Affiliations

  1. Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Chang-Feng Xu, Long Zhang, Jun-Wei Huang, Kai Chen, Shun-Peng Li & Jian-Dong Jiang

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  1. Chang-Feng Xu
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  2. Long Zhang
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  3. Jun-Wei Huang
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  4. Kai Chen
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Corresponding author

Correspondence to Jian-Dong Jiang.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain NK8T is KU877213.

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Xu, CF., Zhang, L., Huang, JW. et al. Aquamicrobium soli sp. nov., a bacterium isolated from a chlorobenzoate-contaminated soil. Antonie van Leeuwenhoek 110, 305–312 (2017). https://doi.org/10.1007/s10482-016-0800-8

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  • DOI: https://doi.org/10.1007/s10482-016-0800-8

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