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Winogradskyella aurantia sp. nov., isolated from a marine solar saltern

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Abstract

A Gram-stain negative, non-flagellated, facultatively anaerobic, slightly halophilic bacterium, WNB302T, was isolated from a marine solar saltern in Wendeng, China (122°0′38.85″E, 36°57′56.49″N). Cells of strain WNB302T were 0.2–0.7 µm wide and 2.0–10.0 µm long, catalase- positive and oxidase-negative. Colonies were opaque, orange and approximately 1.0–2.0 mm in diameter after culture for 96 h on marine agar 2216. Growth occurs at 15–37 °C (optimum, 33–35 °C), pH 6.0–8.5 (optimum, pH 7.0–7.5), and with 0.5–7% NaCl (optimum, 2–3% NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain WNB302T belongs to the genus Winogradskyella and is closely related to Winogradskyella exilis KCTC 32356T. Strain WNB302T exhibited 96.2 and 96.0% 16S rRNA gene sequence similarities with W. exilis KCTC 32356T and W. litoriviva KCTC 23972T. Average nucleotide identity (ANI) value based on draft genomes between strain WNB302T and Winogradskyella thalassocola KCTC 12221T showed a relatedness of 81.1% (with 93.7% of 16S rRNA gene sequence similarity). The major respiratory quinone of strain WNB302T was found to be MK-6, and the dominant fatty acids were found to be iso-C15:0 and iso-C15:1 G. The major polar lipids of strain WNB302T were three unidentified lipids (L1, L2 and L3), one unidentified aminolipids (AL1) and phosphatidylethanolamine (PE). The genomic DNA G+C content was 37.0 mol%. On the basis of the data presented, strain WNB302T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella aurantia sp. nov. is proposed. The type strain is WNB302T (= KCTC 52614T = MCCC 1H00172T).

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References

  • Begum Z, Srinivas TN, Manasa P, Sailaja B, Sunil B, Prasad S, Shivaji S (2013) Winogradskyella psychrotolerans sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from Arctic sediment. Int J Syst Evol Microbiol 63:1646–1652

    Article  CAS  PubMed  Google Scholar 

  • Bernardet JF, Nakagawa Y, Holmes B (2002) Proposal minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070

    CAS  PubMed  Google Scholar 

  • Dong XZ, Cai MY (2001) Determination of biochemical properties. Manual for the systematic identification of general bacteria. Science Press, Beijing, pp 370–398

    Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: 226 a maximum likelihood 227 approach. J Mol Evol 17:368–376

    Article  CAS  PubMed  Google Scholar 

  • Ivanova EP, Christen R, Gorshkova NM, Zhukova NV, Kurilenko VV, Crawford RJ, Mikhailov VV (2010) Winogradskyella exilis sp. nov., isolated from the starfish Stellaster equestris, and emended description of the genus Winogradskyella. Int J Syst Evol Microbiol 60:1577–1580

    Article  CAS  PubMed  Google Scholar 

  • Kang CH, Lee SY, Yoon JH (2013) Winogradskyella litorisediminis sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 63:1793–1799

    Article  CAS  PubMed  Google Scholar 

  • Kim SB, Nedashkovskaya OI (2010) Winogradskyella pacifica sp. nov., a marine bacterium of the family Flavobacteriaceae. Int J Syst Evol Microbiol 60:1948–1951

    Article  CAS  PubMed  Google Scholar 

  • Kim JY, Oh DC (2012) Winogradskyella jejuensis sp. nov., a marine bacterium isolated from a brown alga Carpopeltis affinis. J Microbiol 50:888–892

    Article  CAS  PubMed  Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence data-base with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

  • Kim SJ, Choi YR, Park SJ, Kim JG, Shin KS, Roh DH, Rhee SK (2013) Winogradskyella pulchriflava sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 63:3062–3068

    Article  CAS  PubMed  Google Scholar 

  • Kim JY, Park SH, Seo GY, Kim YJ, Oh DC (2015) Winogradskyella eckloniae sp. nov., a marine bacterium isolated from the brown alga Ecklonia cava. Int J Syst Evol Microbiol 65:2791–2796

    Article  CAS  PubMed  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  PubMed  Google Scholar 

  • Lau SC, Tsoi MM, Li X, Plakhotnikova I, Dobretsov S, Lau KW, Wu M, Wong PK, Pawlik JR, Qian PY (2005) Winogradskyella poriferorum sp. nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas. Int J Syst Evol Microbiol 55:1589–1592

    Article  CAS  PubMed  Google Scholar 

  • Lee SY, Park S, Oh TK, Yoon JH (2012) Winogradskyella aquimaris sp. nov., isolated from seawater. Int J Syst Evol Microbiol 62:1814–1818

    Article  CAS  PubMed  Google Scholar 

  • Lee DH, Cho SJ, Kim SM, Lee SB (2013) Winogradskyella damuponensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 63:321–326

    Article  CAS  PubMed  Google Scholar 

  • Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ (2014) Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 64:2204–2209

    Article  CAS  PubMed  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Nedashkovskaya OI, Kim SB, Han SK, Snauwaert C, Vancanneyt M, Swings J, Kim KO, Lysenko AM, Rohde M, Frolova GM, Mikhailov VV, Bae KS (2005) Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae. Int J Syst Evol Microbiol 55:49–55

    Article  CAS  PubMed  Google Scholar 

  • Nedashkovskaya OI, Vancanneyt M, Kim SB, Zhukova NV (2009) Winogradskyella echinorum sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 59:1465–1468

    Article  CAS  PubMed  Google Scholar 

  • Nedashkovskaya OI, Kukhlevskiy AD, Zhukova NV (2012) Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola, Winogradskyella echinorum, Winogradskyella exilis and Winogradskyella eximia. Int J Syst Evol Microbiol 62:1450–1456

    Article  CAS  PubMed  Google Scholar 

  • Nedashkovskaya OI, Kukhlevskiy AD, Zhukova NV, Kim SJ, Rhee SK, Mikhailov VV (2015) Winogradskyella litoriviva sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol 65:3652–3657

    Article  CAS  PubMed  Google Scholar 

  • Park S, Yoon JH (2013) Winogradskyella undariae sp. nov., a member of the family Flavobacteriaceae isolated from a brown algae reservoir. Antonie Van Leeuwenhoek 104:619–626

    Article  CAS  PubMed  Google Scholar 

  • Park S, Park JM, Won SM, Bae KS, Yoon JH (2014) Winogradskyella wandonensis sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 64:1520–1525

    Article  CAS  PubMed  Google Scholar 

  • Park S, Park JM, Won SM, Yoon JH (2015) Winogradskyella crassostreae sp. nov., isolated from an oyster (Crassostrea gigas). Int J Syst Evol Microbiol 65:2890–2895

    Article  CAS  PubMed  Google Scholar 

  • Pinhassi J, Nedashkovskaya OI, Hagström A, Vancanneyt M (2009) Winogradskyella rapida sp. nov., isolated from protein-enriched seawater. Int J Syst Evol Microbiol 59:2180–2184

    Article  CAS  PubMed  Google Scholar 

  • Reichenbach H (1992) The order Cytophagales. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds) The Prokaryotes. Springer, New York, pp 3631–3675

    Chapter  Google Scholar 

  • Richter M, Rosselló-Móra R (2009) Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 106:19126–19131

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Romanenko LA, Tanaka N, Frolova GM, Mikhailov VV (2009) Winogradskyella arenosi sp. nov., a member of the family Flavobacteriaceae isolated from marine sediments from the Sea of Japan. Int J Syst Evol Microbiol 59:1443–1446

    Article  CAS  PubMed  Google Scholar 

  • Rzhetsky A, Nei M (1993) Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095

    CAS  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI, Inc, Newark, DE

  • Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654

    Google Scholar 

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 25:4876–4882

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yoon JH, Lee SY (2012) Winogradskyella multivorans sp. nov., a polysaccharide-degrading bacterium isolated from seawater of an oyster farm. Antonie Van Leeuwenhoek 102:231–238

    Article  CAS  PubMed  Google Scholar 

  • Yoon BJ, Byun HD, Kim JY, Lee DH, Kahng HY, Oh DC (2011) Winogradskyella lutea sp. nov., isolated from seawater, and emended description of the genus Winogradskyella. Int J Syst Evol Microbiol 61:1539–1543

    Article  CAS  PubMed  Google Scholar 

  • Zhang DC, Liu YX, Huang HJ, Weber K, Margesin R (2016) Winogradskyella sediminis sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 66:3157–3163

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (31370057).

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  1. College of Marine Science, Shandong University at Weihai, Weihai, 264209, China

    Yue Sun, Bi-Yao Chen & Zong-Jun Du

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  1. Yue Sun
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  2. Bi-Yao Chen
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Correspondence to Zong-Jun Du.

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The authors declare that they have no conflict of interest. This article does not contain any studies with animals performed by any of the authors. Informed consent was obtained from all individual participants included in the study.

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Sun, Y., Chen, BY. & Du, ZJ. Winogradskyella aurantia sp. nov., isolated from a marine solar saltern. Antonie van Leeuwenhoek 110, 1445–1452 (2017). https://doi.org/10.1007/s10482-017-0897-4

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