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Deinococcus petrolearius sp. nov. isolated from crude oil recovery water in China

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Abstract

A Gram-stain positive, non-motile, spherical, red-pigmented and facultatively anaerobic bacterium, designated strain 6.1T, was isolated from a crude oil recovery water sample from the Huabei oil field in China. The novel strain exhibited tolerance of UV irradiation (> 1000 J m−2). Based on 16S rRNA gene sequence comparisons, strain 6.1T shows high similarity to Deinococcus citri DSM 24791T (98.1%) and Deinococcus gobiensis I-0T (97.8%), with less than 93.5% similarity to other closely related taxa. The major cellular fatty acids were identified as summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH), followed by iso-C17:1 ω9c and C16:0. The polar lipid profile was found to contain phospholipids, glycolipids, phosphoglycolipids and aminophospholipids. The predominant respiratory quinone was identified as MK-8. The DNA G + C content was determined to be 68.3 mol %. DNA–DNA hybridization between strain 6.1T and D. citri DSM 24791T was 45.6 ± 7.1% and with D. gobiensis I-OT was 36.6 ± 4.7%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, we conclude strain 6.1T represents a novel species of the genus Deinococcus, for which we propose the name Deinococcus petrolearius sp. nov. The type strain is 6.1T (= CGMCC 1.15053T = KCTC 33744T).

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Acknowledgements

We would like to thank Prof. Min Lin of Chinese Academy of Agricultural Sciences, Beijing, China for providing type strain D. gobiensis I-OT to us. This research was funded by the National Natural Science Foundation of China (No. 31400005 and No. 21473256), the Fundamental Research Funds for the Central Universities of China and the Key Research Project of Shandong Province (No. 2017GGX40114).

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Authors and Affiliations

  1. State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering & Biotechnology, China University of Petroleum, 266580, Qingdao, China

    Lijun Xi, Nenghu Qiao, Jingjing Zhang, Jing Li, Dejian Liu & Jianguo Liu

  2. Petroleum Production Engineering Institute of Huabei Oilfield Ltd, 062552, Renqiu, China

    Jing You

  3. School of Chemistry and Molecular Engineering, East China University of Science and Technology, 200237, Shanghai, China

    Jing You

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  1. Lijun Xi
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  2. Nenghu Qiao
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Correspondence to Lijun Xi or Jing Li.

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Xi, L., Qiao, N., Zhang, J. et al. Deinococcus petrolearius sp. nov. isolated from crude oil recovery water in China. Antonie van Leeuwenhoek 111, 353–360 (2018). https://doi.org/10.1007/s10482-017-0957-9

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