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Pseudofrancisella aestuarii gen. nov., sp. nov., a novel member of the family Francisellaceae isolated from estuarine seawater

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Abstract

A Gram-negative, aerobic, non-motile and non-spore forming bacterium, designated strain SYSU WZ-2T, was isolated from an estuarine seawater sample. Growth of strain SYSU WZ-2T was observed at temperature range of 10–40° C (optimum, 32 °C), pH range of 6–10 (optimum, pH 7–8) and in the presence of up to 5.0% NaCl (w/v). The DNA G+C content of the novel strain was determined to be 30.1% (genome). The major polar lipids were found to be diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid, two unidentified aminophospholipids and two unidentified phospholipids. The major fatty acids were C18:0 3-OH (27.5%), C18:1ω9c (19.3%), C16:0 (17.0%) and C14:0 (12.9%). The respiratory quinone was found to be ubiquinone Q8. Pairwise comparison of the 16S rRNA gene sequence showed that strain SYSU WZ-2T shares high identities with members of the genera Francisella (94.8–95.9%) and Allofrancisella (93.8–94.2%). The phylogenetic dendrograms based on 16S rRNA gene sequences with the members of the family Francisellaceae showed that the strain SYSU WZ-2T formed a distinct phylogenetic lineage well separated from the members of the genera Francisella and Allofrancisella. MALDI-TOF mass spectrometric analysis also depicted a different profile for strain SYSU WZ-2T compared with those of members of the genera Francisella and Allofrancisella. Based on the above results and differences in phenotypic and chemotaxonomic features, strain SYSU WZ-2T is characterized to represent a new species of a novel genus, for which the name Pseudofrancisella aestuarii gen. nov., sp. nov. is proposed (type strain SYSU WZ-2T = KCTC 52557T = CGMCC 1.13718T).

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Acknowledgements

Ping-Hua Qu was supported by Research Fund for Outstanding Young in Higher Education Institutions of Guangdong Province (2013 LYM_0015), and Science and Technology Planning Project of Guangdong Province, China (2017A0202150683). Wen-Jun Li was supported by the Guangdong Province Higher Vocational Colleges and Schools Pearl Scholar Founded Scheme (2014).

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Author notes

  1. Min-Ling Zheng and Jian-Yu Jiao have contributed equally to this work.

Authors and Affiliations

  1. The Second Clinic College, Guangzhou University of Chinese Medicine, Guangzhou, 510006, People’s Republic of China

    Min-Ling Zheng, Liang-Hui Li, Cha Chen & Ping-Hua Qu

  2. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Jian-Yu Jiao, Lei Dong, Ming-Xian Han, Min Xiao & Wen-Jun Li

  3. Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, 510006, People’s Republic of China

    Cha Chen & Ping-Hua Qu

  4. College of Fisheries, Henan Normal University, Xinxiang, 453007, People’s Republic of China

    Wen-Jun Li

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Contributions

WJL and PHQ designed research and project outline. MLZ, LD, MXH, LHL and MX performed isolation, deposition and identification. PHQ and CC performed MALDI-TOF–MS analysis. JYJ and WJL performed genome analysis. PHQ, MLZ and WJL drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ping-Hua Qu or Wen-Jun Li.

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Zheng, ML., Jiao, JY., Dong, L. et al. Pseudofrancisella aestuarii gen. nov., sp. nov., a novel member of the family Francisellaceae isolated from estuarine seawater. Antonie van Leeuwenhoek 112, 877–886 (2019). https://doi.org/10.1007/s10482-018-01220-0

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