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Anoxybacillus sediminis sp. nov., a novel moderately thermophilic bacterium isolated from a hot spring

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Abstract

A Gram-stain positive, moderately thermophilic, aerobic, spore-forming and rod-shaped bacterium, designated YIM 73012T, was isolated from a sediment sample collected from a hot spring located in Tibet, China, and was characterized by using a polyphasic taxonomy approach. The strain is oxidase positive and catalase negative. Growth occurred at 37–65 °C (optimum, 45–50 °C), at pH 6.0–8.5 (optimum, pH 7.0–7.5) and with 0.5–3.5% NaCl (optimum, 0.5–1.0%, w/v). The major fatty acids were iso-C15:0, iso-C16:0 and C16:0. The major polar lipids comprised of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine and phosphatidylglycerol. The cell wall peptidoglycan contained meso-diaminopimelic acid. The respiratory quinone was MK-7. The G+C content of genomic DNA was 43.6 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain YIM 73012T forms a distinct lineage with respect to the genus Anoxybacillus in the family Bacillaceae. Based on 16S rRNA gene sequence identities the closely related phylogenetic neighbours are Anoxybacillus caldiproteolyticus DSM 15730T (96.7%) and Saccharococcus thermophilus DSM 4749T (96.6%). Strain YIM 73012T was distinguishable from the closely related reference strains by the differences in phenotypic, chemotaxonomic and genotypic characteristics, and represents a novel species of the genus Anoxybacillus, for which the name Anoxybacillus sp. nov. is proposed. The type species is Anoxybacillus sediminis sp. nov., with the type strain YIM 73012T (= KCTC 33884T = DSM 103835T).

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Acknowledgements

The authors are grateful to Dr. Rüdiger Pukall (DSMZ, Germany) for providing the reference type strains. This work was supported by the Key Project of International Cooperation of Ministry of Science and Technology (MOST, China) (No. 2013DFA31980), Science and Technology Infrastructure work project (No. 2015FY110100), National Natural Science Foundation of China (No. 31470139) and Basic Scientific Research Service Fee Project in Colleges and Universities (No. 17lgjc19). W.-J. Li was also supported by Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme (2014).

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Author notes

  1. Inam Ullah Khan, Neeli Habib and Min Xiao have contributed equally to this work

Authors and Affiliations

  1. Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, People’s Republic of China

    Inam Ullah Khan, Neeli Habib, Xiao-Yang Zhi & Wen-Jun Li

  2. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Min Xiao, Asem Mipeshwaree Devi, Nimaichand Salam & Wen-Jun Li

  3. Department of Biological Sciences, Gomal University, Dera Ismail Khan, KPK, Pakistan

    Inam Ullah Khan

  4. Department of Botany, University of Malakand, Chakdara, Dir Lower, KPK, Pakistan

    Maham Habib

  5. Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Saeid Hejazi

  6. Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Saeid Hejazi

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  1. Inam Ullah Khan
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Contributions

IUK, NH and. W-JL conducted this study. IUK, NH, AMD and. MX performed the experiments. XYZ and W-JL supervised the experiments. IUK, MSH, MH and NS wrote the manuscript. All of the authors assisted in writing the manuscript, discussed the results and commented on the manuscript

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Correspondence to Xiao-Yang Zhi or Wen-Jun Li.

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Khan, I.U., Habib, N., Xiao, M. et al. Anoxybacillus sediminis sp. nov., a novel moderately thermophilic bacterium isolated from a hot spring. Antonie van Leeuwenhoek 111, 2275–2282 (2018). https://doi.org/10.1007/s10482-018-1118-5

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