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In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina

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Abstract

The aim of this work was to adapt and apply the in vitro larval bioassay packet test (LPT) to diagnose resistance of Rhipicephalus microplus ticks to fipronil in Argentina. Ten serial dilutions of fipronil were included in the LPT bioassay. Seven populations of R. microplus from six Argentinean Provinces were evaluated. Samples from Colonia Benítez, Quimilí and Intiyaco were unambiguously classified in the category ‘resistant’. In all these cases the 95% confidence intervals did not overlap with that of the susceptible reference strain and the slope values were lower than the slope value obtained for the susceptible reference strain. The resistance ratio (RR50) values of the populations from Colonia Benítez I and II, Quimilí and Intiyaco were 2.23, 3.49, 6.84 and 3.39, respectively. Populations from El Colorado I and II, Acambuco, Vera and Feliciano were classified as ‘susceptible’ because the 95% confidence intervals overlapped with that of the susceptible reference strain and the RR50 values never exceeded 1.32. The results of these LPT bioassays indicate the presence of fipronil resistant R. microplus populations in Argentina.

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Acknowledgements

This work was supported by INTA (PE-E5-I109), Asociación Cooperadora INTA Rafaela, Agencia Nacional de Promoción Científica y Tecnológica (PICT-2015-550), and Ministerio de Ciencia, Tecnología e Innovación Productiva de la Provincia de Santa Fe (PIO140). We thank Gabriel Simonato and Esteban Puntin for collecting the ticks from Feliciano, and Evelina Tarragona for collecting the ticks from Acambuco.

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Authors and Affiliations

  1. Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Kreder 2805, CP 3080, Esperanza, Santa Fe, Argentina

    Jorgelina Torrents

  2. Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela, CC 22, CP 2300, Rafaela, Santa Fe, Argentina

    Nicolás Morel & Santiago Nava

  3. Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Colonia Benítez, Colonia Benítez, Chaco, Argentina

    Maria V. Rossner

  4. Instituto Nacional de Tecnología Agropecuaria, Agencia de Extensión Rural Garabato, Garabato, Santa Fe, Argentina

    Norberto C. Martínez

  5. Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria El Colorado, El Colorado, Formosa, Argentina

    José R. Toffaletti

  6. Consejo Nacional de Investigaciones Científicas y Técnicas (Conicet), Buenos Aires, Argentina

    Santiago Nava

Authors
  1. Jorgelina Torrents
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  2. Nicolás Morel
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  3. Maria V. Rossner
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  4. Norberto C. Martínez
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  5. José R. Toffaletti
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  6. Santiago Nava
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Correspondence to Santiago Nava.

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Torrents, J., Morel, N., Rossner, M.V. et al. In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina. Exp Appl Acarol 82, 397–403 (2020). https://doi.org/10.1007/s10493-020-00554-7

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