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Improvement of a dry formulation of Pseudomonas protegens SN15-2 against Ralstonia solanacearum by combination of hyperosmotic cultivation with fluidized-bed drying

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Abstract

The application value of Pseudomonas protegens in agriculture has been widely studied, but the manufacturing processes used to produce P. protegens limits its usefulness as a biological control agent because of its poor shelf life due to low stress resistance during processing. We combined hyperosmotic cultivation and fluidized-bed drying to prepare much more stable P. protegens preparations. During drying and cryogenic storage, the viability of hyperosmotic cells was significantly improved compared to that of cells prepared by previous methods. Addition of skim milk provided an excellent protective effect for both hyperosmotic and standard cells. In two field trials, when the concentration of the P. protegens preparation was 0.333% w/v, the biocontrol efficacy against tomato bacterial wilt was more than 80%, which was significantly higher than that of the control provided by the antibiotics zhongshengmycin and streptomycin sulfate. The results show that P. protegens products can be successfully prepared by combination of hyperosmotic cultivation and fluidized-bed drying.

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Acknowledgements

The authors gratefully acknowledge financial support from the National Key Research and Development Program of China (Nos. 2017YFD0200400; 2017YFD0201100) and the Science and Technology Commission of Shanghai Municipality, China (No. 18391902500).

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  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

    Xiaobing Wang, Danyan Tang & Wei Wang

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  1. Xiaobing Wang
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  2. Danyan Tang
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  3. Wei Wang
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Correspondence to Wei Wang.

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Wang, X., Tang, D. & Wang, W. Improvement of a dry formulation of Pseudomonas protegens SN15-2 against Ralstonia solanacearum by combination of hyperosmotic cultivation with fluidized-bed drying. BioControl 65, 751–761 (2020). https://doi.org/10.1007/s10526-020-10042-x

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