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Phylogenetic Relationships and Possible Hybrid Origin of Lycoris Species (Amaryllidaceae) Revealed by ITS Sequences

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To examine interspecific relationships and test the hypothesis of hybrid origin within Lycoris species, this study used data from parsimony analyses with nuclear ITS sequences for 19 taxa representing 14 species of Lycoris and two outgroup taxa. The ITS sequences resolved three infrageneric clades. One clade included L. chinensis, L. longituba, L. longituba var. flava, L. anhuiensis, and L. aurea; the second one consisted of L. sprengeri, L. radiata, L. radiata var. radiata, L. radiata var. pumila, L. haywardii, L. rosea, L. sanguinea var. sanguinea, and L. sanguinea var. koreana; and the third included L. caldwellii, L. straminea, L. albiflora, L. flavescens, and two hybrids. The results strongly support the hypothesis that L. straminea originated from hybridization between L. chinensis and L. radiata var. pumila, and the allotriploid L. caldwellii and L. albiflora derived from hybridization between L. chinensis and L. sprengeri. As nucleotide additivity was observed in the artificial hybrids and several presumed hybrids, the likelihood of hybrid origin of Lycoris species is supported.

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Acknowledgments

We extend special thanks to Dr. Siro Kurita (Chiba University, Japan), Mr. Luhuan Lou, and Mrs. Jinzhen Lin for kindly providing samples of some species in this study. We thank Dr. Alan W. Meerow (National Germplasm Repository, USDA, ARS-SHRS) for providing the ITS sequence of Ungernia flava and giving comments on the manuscript. The study was supported by grants from the National Science Foundation of China (grants 39870079 and 30170062).

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Authors and Affiliations

  1. Laboratory of Systematic and Evolutionary Botany and Biodiversity, College of Life Sciences, Zhejiang University, Hangzhou, 310058, P.R. China

    Shude Shi, Yingxiong Qiu, Enxiang Li & Chengxin Fu

  2. Hangzhou Botanical Garden, Hangzhou, 310013, P.R. China

    Ling Wu

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  1. Shude Shi
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  2. Yingxiong Qiu
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  4. Ling Wu
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Correspondence to Chengxin Fu.

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These authors contributed equally to this work.

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Shi, S., Qiu, Y., Li, E. et al. Phylogenetic Relationships and Possible Hybrid Origin of Lycoris Species (Amaryllidaceae) Revealed by ITS Sequences. Biochem Genet 44, 198–208 (2006). https://doi.org/10.1007/s10528-006-9023-4

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