Abstract
Production of the benzophenanthridine alkaloids in Eschscholtzia californica suspension cell cultures was optimized by adding 0.5 mg methyl jasmonate (MJ) and 0.02 mg salicylic acid (SA)/g FCW after 7 days cultivation. Sanguinarine reached 24 mg/g DCW by such treatment; 10 times higher than in control cell cultures. MJ and SA induced expression of berberine bridge enzyme and 3′-hydroxy-(S)-N-methylcoclaurine-4′-O-methyltransferase, respectively. MJ plus SA induced over-expression of both enzymes.
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This work was financially supported by the Korea Science and Engineering Foundation (KOSEF)through the Advanced Environmental Biotechnology Research Center (ABERC) at POSTECH.
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Cho, HY., Lee-Parsons, C.W.T., Yoon, SY.H. et al. Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures. Biotechnol Lett 29, 2001–2005 (2007). https://doi.org/10.1007/s10529-007-9469-4
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DOI: https://doi.org/10.1007/s10529-007-9469-4