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Fungal endophytes-induced abscisic acid is required for flavonoid accumulation in suspension cells of Ginkgo biloba

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Abstract

Treatment of suspension cells of Ginkgo biloba with fungal endophytes resulted in accumulation of flavonoids, increased abscisic acid (ABA) production and activation of phenylalanine ammonia-lyase (PAL). Fluridone, an inhibitor of ABA biosynthesis, was effective in inhibiting fungal endophytes-induced ABA biosynthesis, increase of PAL activity and flavonoids accumulation. Moreover, exogenous application of ABA enhanced PAL activity and increased accumulation of flavonoids in G. biloba cells with or without fungal endophytes elicitor. These finding suggest a causal relationship between ABA release and both PAL activity and flavonoid accumulation under fungal endophytes treatment and that ABA is involved in fungal endophytes-induced flavonoids accumulation in this plant.

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Acknowledgments

This work is supported by the research fund for the doctoral degree scholars of Taishan Medical University (No. 2005-04).

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Authors and Affiliations

  1. Department of Biochemistry, Taishan Medical University, 271000, Tai’an, People’s Republic of China

    Gangping Hao & Haiwei Ji

  2. College of Life Sciences, Shandong Normal University, 250014, Jinan, People’s Republic of China

    Xihua Du

  3. Department of Pharmacy, Jiangxi College of Traditional Chinese Medical, 344000, Fuzhou, People’s Republic of China

    Faxing Zhao

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  1. Gangping Hao
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  2. Xihua Du
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  3. Faxing Zhao
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  4. Haiwei Ji
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Correspondence to Gangping Hao.

Additional information

Gangping Hao and Xihua Du contributed equally to this paper.

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Hao, G., Du, X., Zhao, F. et al. Fungal endophytes-induced abscisic acid is required for flavonoid accumulation in suspension cells of Ginkgo biloba . Biotechnol Lett 32, 305–314 (2010). https://doi.org/10.1007/s10529-009-0139-6

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  • DOI: https://doi.org/10.1007/s10529-009-0139-6

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