Abstract
The effects of photoperiod on the development of in vitro grown plantlets of yam (Dioscorea alata L.), were investigated. Plantlets were transplanted into pots, acclimatizated until they reached vegetative stages V1 (3 leaves) or V2 (8 leaves), and then grown under 12-h or 16-h photoperiod. The formation and development of underground tubers was only induced under 12-h photoperiod. Tuber initiation was not related to the initial vegetative stage of plants, and the tubers were visible at about 18 – 24 d. On the contrary, a 16-h photoperiod inhibited tuber formation and stimulated vine and leaf growth. The total dry matter production and the number of leaves per plant of V1 stage plants were 50 and 30 % lower respectively, after 44 d under 12-h compared to 16-h photoperiod. These parameters were not influenced by photoperiod in V2 stage plants. Consequently, the effect of 12-h photoperiod on dry matter of V1 plants was attributed to a source limitation related to the early initiation of tuberization. The transfer of plants grown under 12-h to 16-h photoperiod stopped tuber growth and starch accumulation. On the other hand, it stimulated the shoots and the roots to grow.
Abstract
Abbreviations
- LD:
-
long day (16 h)
- SH:
-
short day (12 h)
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References
Alvarenga, A.A., Valio, I.F.M.: Influence of temperature and photoperiod on flowering and tuberous root formation of Pachyrhizus tuberosus.-Ann. Bot. 64: 411–414, 1989.
Bergmeyer, H.U., Bernt, E., Schmidt, F., Stork, H.: D-glucose: determination with hexokinase and glucose 6-phosphate dehydrogenase.-In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Pp. 1196–1201. Verlag-Chemie/Academic Press, New York and London 1974.
Chapman, T.: Some investigations into factors limiting yields of the white Lisbon yam (Dioscorea alata L.) under Trinidad conditions.-Trop. Agr. 42: 145–151, 1965.
Enyi, B.A.C.: The effects of seed size and spacing on growth and yield of lesser yam Dioscorea esculenta.-J. agr. Sci. 78: 215–225, 1972.
Ewing, E.E., Wareing, P.F.: Shoot, stolon, and tuber formation of potato (Solanum tuberosum L.) cuttings in response to photoperiod.-Plant Physiol. 61: 348–353, 1978.
Ferguson, T.U., Haynes, P.H., Spence, J.A.: Distribution of dry matter and mineral nutrients in tuber of two cultivars of Dioscorea alata L.-Trop. Agr. 57: 61–67, 1980.
Hayashi, M., Ishihata, K.: Studies on the development and the thickening growth of yam (Dioscorea spp.) tubers. 2. Effects of photoperiod and temperature on growth and enlargement of tubers.-Jap. J. trop. Agr. 35(2): 79–83, 1991.
Lacointe, A., Zinsou, C.: Effets de la date de plantation sur la croissance et le developpement de plantules d’igname (Dioscorea alata L.) produites par culture in vitro.-Agronomie 7: 475–481, 1987.
Lian, M.L., Chakrabarty, D., Paek, K.Y.: Bublet formation from bulbscale segments of Lilium using bioreactor system.-Biol. Plant. 46: 199–203, 2003.
Mantell, S.H., Hugo, S.A.: Effects of photoperiod, mineral medium strength, inorganic ammonium, sucrose and cytokinin on root, shoot and microtuber development in shoot cultures of Dioscorea alata L. and D. bulbifera L. yams.-Plant Cell Tissue Organ Cult. 16: 23–37, 1989.
Mead, R., Curnow, R.N.: Statistical Methods in Agriculture and Experimental Biology.-Chapman and Hall, London 1983.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture.-Physiol. Plant. 15: 473–479, 1962.
Okubo, H., Masunaga, T., Yamashita, H., Uemoto, S.: Effects of photoperiod and temperature on tuberous root formation in winged bean (Psophocarpus tetragonolobus).-Sci. Hort. 49: 1–8, 1992.
Onwuene, I.C.: Sett weight effects on time of tuber formation, and on tuber yield characteristics, in water yam (Dioscorea alata L.).-J. agr. Sci. 91: 317–319, 1978.
Orkwor, G.C., Ekanayake, I.J.: Food yams.-In: Orkwor, G.C. Asiedu, R., Ekanayake, I.J. (ed.): Advances in Research. Pp. 39–62, IITA/NRCRI, Nigeria 1998.
Rodriguez, W.: Crop Physiology of the Greater Yam (Dioscorea alata L.).-Verlag Ulrich E. Grauer, Stuttgart 1997.
Shiwachi, H., Ayankanmi, T., Asiedu, R.: Effect of day length on the development of tubers in yams (Dioscorea spp.). Trop. Sci. 42: 162–170, 2002.
Sladky, Z., Bartosova, L.: In vitro induction of axillary potato microtubers and improvement of their quality.-Biol. Plant. 32: 181–188, 1990.
Snyder, R.G., Ewing, E.E.: Interactive effects of temperature, photoperiod, and cultivar on tuberization of potato cuttings.-HortScience 24: 336–338, 1989.
Struik, P.C., Van Heusden, E., Burger-Meijer, K.: Effects of short periods of long days on the development, yield and size distribution of potato tubers.-Neth. J. agr. Sci. 36: 11–22, 1988.
Vaillant, V., Desfontaines, L.: Assimilate partitioning in Pachyrhizus erosus tubers under short days.-Physiol. Plant. 93: 558–562, 1995.
Yoshida, Y., Kanahama, K.: Effects of photoperiod and temperature on the development of spikes and new tubers in Chinese yam (Dioscorea opposita Thunb. cv. Ichoimo).-J. jap. Soc. hort. Sci. 68: 124–129, 1999.
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The present study was supported by the Regional Council of Guadeloupe.
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Vaillant, V., Bade, P. & Constant, C. Photoperiod affects the growth and development of yam plantlets obtained by in vitro propagation. Biol Plant 49, 355–359 (2005). https://doi.org/10.1007/s10535-005-0007-8
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DOI: https://doi.org/10.1007/s10535-005-0007-8