Skip to main content
SpringerLink
Log in

Staining of endothelial cells does not change the result of cell density

  • Short Communication
  • Published:
Cell and Tissue Banking Aims and scope Submit manuscript

Abstract

To determine the corneal endothelial cell density human donor corneas are usually placed in hypotonic balanced salt solution for unstained cell counting. Experimental studies often stain the corneal endothelium to highlight cells and cell borders and simplify counting. However, staining normally terminates the experiments. Up to date, there is no publication comparing endothelial cell counts before and after staining. This study compared the counting results of unstained (hypotonic balanced salt solution) and stained (trypan blue & alizarin red S) assessment of the corneal endothelial cell density. No evidence for significantly differing cell counts was found between unstained and stained analysis of the corneal endothelial cell density. Therefore, we consider unstained counting non-inferior to stained counting.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Kunzmann BC et al (2018) Establishment of a porcine corneal endothelial organ culture model for research purposes. Cell Tissue Bank 19(3):269–276

    Article  CAS  PubMed  Google Scholar 

  • Meltendorf C et al (2007) Endothelial cell density in porcine corneas after exposure to hypotonic solutions. Graefes Arch Clin Exp Ophthalmol 245(1):143–147

    Article  PubMed  Google Scholar 

  • Schroeter J (2009) Endothelial evaluation in the Cornea Bank. Dev Ophthalmol 43:47–62

    Article  PubMed  Google Scholar 

  • Taylor MJ, Hunt CJ (1981) Dual staining of corneal endothelium with trypan blue and alizarin red S: importance of pH for the dye-lake reaction. Br J Ophthalmol 65(12):815–819

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Ophthalmology, University Medical Center Hamburg-Eppendorf (UKE), Martinistraße 52, 20246, Hamburg, Germany

    Daniel A. Wenzel, Martin S. Spitzer & Maximilian Schultheiss

  2. Center of Ophthalmology, University Eye Hospital Tübingen, Elfriede-Aulhorn-Str. 7, Tübingen, Germany

    Berenike C. Kunzmann

Authors
  1. Daniel A. Wenzel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Berenike C. Kunzmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Martin S. Spitzer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maximilian Schultheiss
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maximilian Schultheiss.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wenzel, D.A., Kunzmann, B.C., Spitzer, M.S. et al. Staining of endothelial cells does not change the result of cell density. Cell Tissue Bank 20, 327–328 (2019). https://doi.org/10.1007/s10561-019-09759-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10561-019-09759-6

Keywords

Search

Navigation