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Antimicrobial activity of sugar beet lignocellulose films containing tung oil and cedarwood essential oil

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Abstract

Several studies have been reported on the use of cellulose, lignocellulose, chitin and other biological macromolecules for food packaging. One of the major drawbacks limiting their wide uses is their limited antimicrobial and water vapor barrier properties. In this study, cedarwood (Thuja occidentalis) essential oil (CWO), known to contain terpenes, polyphenols and tung oil, known to contain fatty acids, were used as additives to improve water vapor barrier, antimicrobial, and physical properties of laboratory cast lignocellulose films. Physicochemical and mechanical properties were tested to evaluate the impact of the addition of oils in the films formulations. The addition of 15 % CWO and tung oils improved water vapor permeability by more than 25 % as also evidenced by the increase of contact angle between water and film by 134 % from 38.98° to 89.36° with 15 % tung oil. FTIR was used to monitor the presence of hydrophobic groups at 1463 and 1741 cm−1 from the oil on the film spectra confirming the improvement of film hydrophobicity from oils interactions. However, the addition of 15 % hydrophobic oils promoted a significant decrease in tensile properties of films (p ≤ 0.05) by 40 % for CWO and 32 % for tung oil, respectively, due to a partial incompatibility between hydrophilic lignocellulose and hydrophobic oils. The use of a tailored coupling agent to reduce the incompatibility and to improve the load charge transfer between hydrophobic and hydrophilic group might reduce the decrease of the tensile properties with the addition of hydrophobic compounds. No significant difference (p > 0.05) was observed on the thermal stability of films using TGA. The antimicrobial effects of lignocellulose films against bacteria namely Listeria innocua, Escherichia coli, and Salmonella enterica was tested by disk inhibition zone method and showed an improvement with the addition of 5 % CWO in the film. The Microbe Growth Index of the lignocellulose cellulosic films containing 20 % w/w of CWO decreases by 80 %. CWO and tung oil are good candidates to control microbial growth and water vapor permeability in flexible films. Future work will focus on the olfactory and tensile properties for applications in food packaging.

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References

  • Adams R (1991) Cedar wood oil—Analyses and properties. Essential oils and waxes. Springer, Berlin, pp 159–173

    Chapter  Google Scholar 

  • Ahmad M, Benjakul S, Prodpran T, Agustini TW (2012) Physico-mechanical and antimicrobial properties of gelatin film from the skin of unicorn leatherjacket incorporated with essential oils. Food Hydrocolloids 28:189–199

    Article  CAS  Google Scholar 

  • ASTM Standard (2012) Standard test method for tensile properties of thin plastic sheeting. ASTM International, West Conshohocken, PA, 2003, doi: 10.1520/D0882-12, www.astm.org

  • Atkins P, De Paula J (2010) Physical chemistry for the life sciences. Oxford University Press, Oxford

    Google Scholar 

  • Berndt S, Wesarg F, Wiegand C, Kralisch D, Müller FA (2013) Antimicrobial porous hybrids consisting of bacterial nanocellulose and silver nanoparticles. Cellulose 20:771–783

  • Bezić N, Skočibušić M, Dunkić V, Radonić A (2003) Composition and antimicrobial activity of Achillea clavennae L. essential oil. Phytother Res 17:1037–1040

    Article  Google Scholar 

  • Brown K, Keeler W (2005) The history of tung oil. Wildland Weeds 9:4–24

    Google Scholar 

  • Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 94:223–253

    Article  CAS  Google Scholar 

  • Cinelli P, Schmid M, Bugnicourt E, Wildner J, Bazzichi A, Anguillesi I, Lazzeri A (2014) Whey protein layer applied on biodegradable packaging film to improve barrier properties while maintaining biodegradability. Polym Degrad Stab 108:151–157

    Article  CAS  Google Scholar 

  • Couladis M, Chinou I, Tzakou O, Petrakis P (2003) Composition and antimicrobial activity of the essential oil of Hypericum rumeliacum subsp. apollinis (Boiss. & Heldr.). Phytother Res 17:152–154

    Article  CAS  Google Scholar 

  • Feng J, Shi Q, Li W, Shu X, Chen A, Xie X, Huang X (2014) Antimicrobial activity of silver nanoparticles in situ growth on TEMPO-mediated oxidized bacterial cellulose. Cellulose 21:4557–4567

    Article  CAS  Google Scholar 

  • Fernández-Pan I, Mendoza M, Maté JI (2013) Whey protein isolate edible films with essential oils incorporated to improve the microbial quality of poultry. J Sci Food Agric 93:2986–2994

    Article  Google Scholar 

  • Fishman ML, Chau HK, Coffin DR, Cooke PH, Qi P, Yadav MP, Hotchkiss AT Jr (2011) Physico-chemical characterization of a cellulosic fraction from sugar beet pulp. Cellulose 18:787–801

    Article  CAS  Google Scholar 

  • Ghanem S, Olama Z (2014) Antimicrobial potential of lebanese cedar extract against human pathogens and food spoilage microorganisms. Eur J Bot Plant Sci Phytol 13-26

  • Guerrini A (2011) Antiproliferative and erythroid differentiation activities of Cedrus libani seed extracts against K562 human chronic myelogenus leukemia cells. Int J Pharm Biol Arch 2:1658–1662

  • Hall IH, Lee K-H, Mar EC, Starnes CO, Waddell TG (1977) Antitumor agents. 21. A proposed mechanism for inhibition of cancer growth by tenulin and helenalin and related cyclopentenones. J Med Chem 20:333–337

    Article  CAS  Google Scholar 

  • Hammer KA, Carson C, Riley T (1999) Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol 86:985–990

    Article  CAS  Google Scholar 

  • Hernandez E (1994) Edible coatings from lipids and resins. In: Edible coatings and films to improve food quality. CRC Press, Florida, pp 279–303

  • Johnston W, Karchesy J, Constantine G, Craig A (2001) Antimicrobial activity of some Pacific Northwest woods against anaerobic bacteria and yeast. Phytother Res 15:586–588

    Article  CAS  Google Scholar 

  • Kamatou G, Viljoen A, Özek T, Başer K (2010) Chemical composition of the wood and leaf oils from the “Clanwilliam Cedar” (Widdringtonia cedarbergensis JA Marsh): a critically endangered species. S Afr J Bot 76:652–654

    Article  CAS  Google Scholar 

  • Li F, Larock RC (2000) Thermosetting polymers from cationic copolymerization of tung oil: synthesis and characterization. J Appl Polym Sci 78:1044–1056

    Article  CAS  Google Scholar 

  • Lin Z, Renneckar S, Hindman DP (2008) Nanocomposite-based lignocellulosic fibers 1. Thermal stability of modified fibers with clay-polyelectrolyte multilayers. Cellulose 15:333–346

    Article  CAS  Google Scholar 

  • Mosiewicki MA, Aranguren MI (2013) A short review on novel biocomposites based on plant oil precursors. Eur Polym J 49:1243–1256

    Article  CAS  Google Scholar 

  • Oh SY et al (2005) Crystalline structure analysis of cellulose treated with sodium hydroxide and carbon dioxide by means of X-ray diffraction and FTIR spectroscopy. Carbohydr Res 340:2376–2391

    Article  CAS  Google Scholar 

  • Ojagh SM, Rezaei M, Razavi SH, Hosseini SMH (2010) Development and evaluation of a novel biodegradable film made from chitosan and cinnamon essential oil with low affinity toward water. Food Chem 122:161–166

  • Olsson A-M, Salmén L (2004) The association of water to cellulose and hemicellulose in paper examined by FTIR spectroscopy. Carbohydr Res 339:813–818

    Article  CAS  Google Scholar 

  • Özdemir F, Serin ZO, Mengeloğlu F (2013) Utilization of red pepper fruit stem as reinforcing filler in plastic composites. BioResources 8:5299–5308

    Google Scholar 

  • Panten J, Bertram HJ, Surburg H (2004) New woody and ambery notes from cedarwood and turpentine oil. Chem Biodivers 1:1936–1948

    Article  CAS  Google Scholar 

  • Pereda M, Aranguren MI, Marcovich NE (2010) Caseinate films modified with tung oil. Food Hydrocolloids 24:800–808

    Article  CAS  Google Scholar 

  • Prabuseenivasan S, Jayakumar M, Ignacimuthu S (2006) In vitro antibacterial activity of some plant essential oils. BMC Complement Altern Med 6:39

    Article  Google Scholar 

  • Pranoto Y, Salokhe VM, Rakshit SK (2005) Physical and antibacte rial properties of alginate-based edible film incorporated with garlic oil. Food Res Int 38(3):267–272

    Article  CAS  Google Scholar 

  • Regnault-Roger C (1997) The potential of botanical essential oils for insect pest control. Integr Pest Manag Rev 2:25–34

    Article  Google Scholar 

  • Rojas-Graü MA et al (2007) Effects of plant essential oils and oil compounds on mechanical, barrier and antimicrobial properties of alginate–apple puree edible films. J Food Eng 81:634–641

    Article  Google Scholar 

  • Salmieri S, Lacroix M (2006) Physicochemical properties of alginate/polycaprolactone-based films containing essential oils. J Agric Food Chem 54:10205–10214

    Article  CAS  Google Scholar 

  • Salmieri S et al (2014) Antimicrobial nanocomposite films made of poly (lactic acid)–cellulose nanocrystals (PLA–CNC) in food applications—part B: effect of oregano essential oil release on the inactivation of Listeria monocytogenes in mixed vegetables. Cellulose 21:4271–4285

    Article  CAS  Google Scholar 

  • Seydim A, Sarikus G (2006) Antimicrobial activity of whey protein based edible films incorporated with oregano, rosemary and garlic essential oils. Food Res Int 39:639–644

    Article  CAS  Google Scholar 

  • Sharma V, Kundu P (2006) Addition polymers from natural oils—a review. Prog Polym Sci 31:983–1008

    Article  CAS  Google Scholar 

  • Shen Z, Kamdem DP (2015) Development and characterization of biodegradable chitosan films containing two essential oils. Int J Biol Macromol 74:289–296

    Article  CAS  Google Scholar 

  • Shen Z, Ghasemlou M, Kamdem DP (2015) Development and compatibility assessment of new composite film based on sugar beet pulp and polyvinyl alcohol intended for packaging applications. J Appl Polym Sci. doi:10.1002/app.41354

  • Shojaee-Aliabadi S et al (2013) Characterization of antioxidant-antimicrobial κ-carrageenan films containing Satureja hortensis essential oil. Int J Biol Macromol 52:116–124

    Article  CAS  Google Scholar 

  • Tongnuanchan P, Benjakul S, Prodpran T (2013) Physico-chemical properties, morphology and antioxidant activity of film from fish skin gelatin incorporated with root essential oils. J Food Eng 117:350–360

    Article  CAS  Google Scholar 

  • Trumbo D, Mote B (2001) Synthesis of tung oil—diacrylate copolymers via the Diels–Alder reaction and properties of films from the copolymers. J Appl Polym Sci 80:2369–2375

    Article  CAS  Google Scholar 

  • Tunalier Z, Kirimer N, Baser K (2004) A potential new source of cedarwood oil: juniperus foetidissima Willd. J Essent Oil Res 16:233–235

    Article  CAS  Google Scholar 

  • Türünç O, Meier MA (2013) The thiol-ene (click) reaction for the synthesis of plant oil derived polymers. Eur J Lipid Sci Technol 115:41–54

    Article  Google Scholar 

  • Twede D, Selke SE, Kamdem D-P, Shires D (2014) Cartons, crates and corrugated board: handbook of paper and wood packaging technology. DEStech Publications Inc, Lancaster

    Google Scholar 

  • Yang H, Yan R, Chen H, Lee DH, Zheng C (2007) Characteristics of hemicellulose, cellulose and lignin pyrolysis. Fuel 86:1781–1788

    Article  CAS  Google Scholar 

  • Zhao H, Baker GA (2013) Ionic liquids and deep eutectic solvents for biodiesel synthesis: a review. J Chem Technol Biotechnol 88:3–12

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Special thanks go to Michigan Sugar Company for generously offering raw material for this research and to Mr. Alnughaymishi for the agar diffusion and dilution test.

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  1. School of Packaging, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI, 48824, USA

    Zhu Shen & Donatien Pascal Kamdem

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  1. Zhu Shen
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  2. Donatien Pascal Kamdem
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Correspondence to Donatien Pascal Kamdem.

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Shen, Z., Kamdem, D.P. Antimicrobial activity of sugar beet lignocellulose films containing tung oil and cedarwood essential oil. Cellulose 22, 2703–2715 (2015). https://doi.org/10.1007/s10570-015-0679-y

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