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Low genetic differentiation despite high fragmentation in the endemic serpentinophyte Minuartia smejkalii (M. verna agg., Caryophyllaceae) revealed by RADSeq SNP markers

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Abstract

Minuartia smejkalii is an obligate serpentinophyte plant endemic to the Czech Republic. Since the 1960s, the species’ habitat has undergone strong human-mediated fragmentation, resulting in extinction of some populations and dramatic size reduction of the remaining populations. Thus, contrary to the typically stable serpentine habitats, M. smejkalii habitats underwent a recent and severe decline, which can exacerbate the effects of fragmentation on population genetic structure. We examined the genetic structure of all known M. smejkalii populations and two populations of M. corcontica and M. caespitosa, which are closely related, using RADSeq. The results indicate low, but clear differentiation among the three species, thus supporting the status of M. smejkalii as an independent taxon, though more extensive analysis of the whole group is needed. We further show high genetic diversity within M. smejkalii populations, low to moderate among-populations differentiation, and moderate regional differentiation. This could be due to the outcrossing mating system of M. smejkalii promoting high levels of gene flow and historical factors (multiple founder events, a recent bottleneck and/or a genetic time lag). We finally demonstrate that 2–3% of the markers show differentiation patterns consistent with divergent selection, suggesting that some local adaptation might have occured in M. smejkalii. Based on our observations, but without any experimental testing for local adaptation, if a conservation action is to be carried out, we recommend strictly separating the material from the two regions, and if possible, separating the populations within a region.

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Data availability

Genomic data is available at BioSample, ID: SUB5620362.

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Acknowledgements

The study was supported by Project LIFE for Minuartia (Grant No. LIFE15NAT/CZ/000818), which is implemented with the financial contribution of the European Union, the Life program and with the financial contribution of the Ministry of Environment of the Czech Republic and party by and the long-term research development Project RVO 67985939.

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  1. Department of Biology and Ecology, Faculty of Science, University of Ostrava, Ostrava, Czech Republic

    Bojana Stojanova

  2. Institute of Botany, Academy of Sciences of the Czech Republic, Průhonice, Czech Republic

    Maria Šurinová, Vojtěch Zeisek, Zuzana Münzbergová & Hana Pánková

  3. Department of Botany, Faculty of Science, Charles University, Prague, Czech Republic

    Maria Šurinová, Vojtěch Zeisek & Zuzana Münzbergová

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  1. Bojana Stojanova
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  2. Maria Šurinová
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Correspondence to Bojana Stojanova.

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10592_2019_1239_MOESM1_ESM.pdf

Supplementary material 1—BayeScan results of the detection of outlier loci. FST – the locus component of the FST estimate under a multinomial Dirichlet model, q-value – posterior probability of the selection model after correction for multiple testing. (PDF 361 kb)

10592_2019_1239_MOESM2_ESM.pdf

Supplementary material 2—Boxplot of the depth of coverage of the 12 000RADSeq markers initially developed for the study. (PDF 504 kb)

10592_2019_1239_MOESM3_ESM.pdf

Supplementary material 3—BIC for each number of K estimated for the K-mean clustering of the entire data set (M. smejkalii, M. corcontica, and M. caespitosa). (PDF 616 kb)

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Stojanova, B., Šurinová, M., Zeisek, V. et al. Low genetic differentiation despite high fragmentation in the endemic serpentinophyte Minuartia smejkalii (M. verna agg., Caryophyllaceae) revealed by RADSeq SNP markers. Conserv Genet 21, 187–198 (2020). https://doi.org/10.1007/s10592-019-01239-4

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